Bacterial lipases and chronic blepharitis.
Bacterial lipases and chronic blepharitis.
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DOI：
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发表时间：
1986-04
影响因子：
4.4
通讯作者：
J. Dougherty;J. McCulley
中科院分区：
文献类型：
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作者：
J. Dougherty;J. McCulley
对36例正常人和6组60例慢性眼炎患者的眼睑和结膜进行了需氧和厌氧菌培养。最常见的菌种是凝固酶阴性葡萄球菌（C-NS）和痤疮丙酸杆菌。所有这些菌株和所有分离的金黄色葡萄球菌菌株都进行了分解甘油三酯、胆固醇酯和脂肪蜡的能力测试。每个菌株在营养培养基中与适当的基质单独孵育。然后提取每种培养基，并通过薄层色谱法检测底物水解产物的存在。确定了每个个体能够水解特定底物的菌株的百分比。金黄色葡萄球菌是一个一致的和强大的脂肪酶生产者，能够水解所有三种底物。痤疮杆菌能够水解三油酸酯和油酸酯，但不能水解油酸胆固醇。痤疮P.或金黄色葡萄球菌组间无差异。C-NS表现出高度的应变变异性。83%的C-NS菌株能水解三油酸酯，82%能水解油酸酚酯，40%能水解油酸胆固醇。在每个个体分离的脂肪酶阳性C-NS菌株百分比方面，组间差异显著。混合葡萄球菌/脂溢性、睑板性脂溢性、继发性睑板炎和睑板性角膜结膜炎（MKC）组患者比正常人携带更多能够水解油酸胆固醇的C-NS菌株。睑板脂溢性、继发性睑板炎和MKC组的患者比正常人携带更多能够水解油酸behenyl的C-NS菌株。三油酸水解C-NS菌株组间无组间差异。
Eyelids and conjunctivae of 36 normal individuals and 60 patients from six clinical groups of chronic blepharitis were cultured for aerobic and anaerobic bacteria. The most common species isolated were coagulase-negative staphylococci (C-NS) and Propionibacterium acnes. All strains of these species, and all Staphylococcus aureus strains isolated were tested for the ability to break down triglycerides, cholesterol esters, and fatty waxes. Each strain was incubated independently with appropriate substrates in nutrient media. Each medium was then extracted and assayed for the presence of substrate hydrolysis products by thin-layer chromatography. The percentage of strains capable of hydrolyzing a particular substrate was determined for each individual. S. aureus was a consistent and strong lipase producer, able to hydrolyze all three substrates. P. acnes was able to hydrolyze triolein and behenyl oleate but not cholesteryl oleate. No differences were observed among groups for P. acnes or S. aureus. C-NS showed a high degree of strain variability. Eighty-three percent of C-NS strains could hydrolyze triolein, 82% behenyl oleate, and 40% cholesteryl oleate. Significant group differences were seen in the percentage of lipase positive C-NS strains isolated per individual. Patients in the mixed staphylococcal/seborrheic, meibomian seborrheic, secondary meibomitis, and the meibomian keratoconjunctivitis (MKC) groups harbored significantly more C-NS strains capable of hydrolyzing cholesteryl oleate than did normal individuals. Patients in the meibomian seborrheic, secondary meibomitis, and MKC groups harbored significantly more C-NS strains capable of hydrolyzing behenyl oleate than did normals. No group differences were seen among groups with triolein hydrolyzing C-NS strains.