A physical map of the mouse H2 Bat5/Db interval.
A physical map of the mouse H2 Bat5/Db interval.
复制标题
小鼠 H2 Bat5/Db 间隔的物理图。
DOI：
10.1007/s003359900343
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Nakamura,I
中科院分区：
文献类型：
--
作者：
Dagenais,SL;Nakamura,I
用随机引物探针筛选B10基因组DNA的pWE 15粘粒文库，所述探针从人BAT 2、BAT 3、BAT 4和BAT 5基因cDNA制备。分离跨越Bat 5/B144区间的重叠粘粒克隆，并定位10种内切酶的限制性位点。与已发表的BALB/c小鼠相同间隔的长距离限制性酶切图谱相比，B10图谱鉴定出MluI的2个额外识别位点，NotI和NruI各1个。两个图谱之间的差异是由于总基因组DNA中CpG二核苷酸的甲基化和克隆DNA中甲基化的缺失。
A pWE15 cosmid library of B10 genomic DNA was screened with random-primed probes prepared from human cDNAs for the BAT2, BAT3, BAT4 and BAT5 genes. Overlapping cosmid clones spanning theBat5/B144interval were isolated, and mapped for restriction sites of 10 endonucleases. The B10 map identified 2 additional recognition sites forMluI and 1 each forNotI andNruI compared with the published long-range restriction map of the same interval for BALB/c mice. The differences between the 2 maps were due to methylation of CpG dinucleotides in total genomic DNA and absence of methylation in cloned DNA.