Electroacupuncture Facilitates the Integration of Neural Stem Cell-Derived Neural Network with Transected Rat Spinal Cord
Electroacupuncture Facilitates the Integration of Neural Stem Cell-Derived Neural Network with Transected Rat Spinal Cord
复制标题
电针促进神经干细胞衍生的神经网络与横断大鼠脊髓的整合
DOI：
10.1016/j.stemcr.2018.12.015
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发表时间：
2019-02-12
影响因子：
5.9
通讯作者：
Zeng, Yuan-Shan
中科院分区：
文献类型：
--
作者：
Jin, Hui;Zhang, Yu-Ting;Zeng, Yuan-Shan
受损脊髓的恶劣环境使得在用于重建脊髓回路的移植组织工程神经网络中实现更高的生存能力具有挑战性。在这里，我们调查是否可以通过电针（EA），通过改善微环境促进NT-3和TRKC基因过表达的神经干细胞衍生的神经网络支架（NN）移植到横断脊髓内的细胞存活和突触传递。我们的研究结果表明，EA促进移植NN的细胞存活，神经元分化和突触形成。伪狂犬病病毒示踪表明，电针加强了移植神经元与宿主神经回路的突触整合。联合治疗还促进轴突再生、脊髓传导性和功能恢复。研究结果强调EA作为一种潜在的和安全的补充治疗策略，以加强移植NN的存活和突触发生，作为神经元中继，以桥接受损脊髓的两个切断端。
The hostile environment of an injured spinal cord makes it challenging to achieve higher viability in a grafted tissue-engineered neural network used to reconstruct the spinal cord circuit. Here, we investigate whether cell survival and synaptic transmission within an NT-3 and TRKC gene-overexpressing neural stem cell-derived neural network scaffold (NN) transplanted into transected spinal cord could be promoted by electroacupuncture (EA) through improving the microenvironment. Our results showed that EA facilitated the cell survival, neuronal differentiation, and synapse formation of a transplanted NN. Pseudorabies virus tracing demonstrated that EA strengthened synaptic integration of the transplanted NN with the host neural circuit. The combination therapy also promoted axonal regeneration, spinal conductivity, and functional recovery. The findings highlight EA as a potential and safe supplementary therapeutic strategy to reinforce the survival and synaptogenesis of a transplanted NN as a neuronal relay to bridge the two severed ends of an injured spinal cord.