A specific groove design for individualized healing in a canine partial sternal defect model by a polycaprolactone/hydroxyapatite scaffold coated with bone marrow stromal cells.
A specific groove design for individualized healing in a canine partial sternal defect model by a polycaprolactone/hydroxyapatite scaffold coated with bone marrow stromal cells.
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DOI：
10.1002/jbm.a.35012
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发表时间：
2014-10
期刊：
影响因子：
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通讯作者：
Yiwen Xuan;Hua Tang;Bin Wu;Xinyu Ding;Zhong-shan Lu;Wei Li;Zhi-fei Xu
中科院分区：
文献类型：
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作者：
Yiwen Xuan;Hua Tang;Bin Wu;Xinyu Ding;Zhong-shan Lu;Wei Li;Zhi-fei Xu
胸骨缺损的重建在临床上对胸外科医生来说仍然是一个挑战。在这里，我们的目的是探索在大型动物模型中使用新的可生物降解材料进行部分胸骨缺损的个体化重建。我们采用熔融沉积建模（FDM）技术制备具有个性化凹槽的聚己内酯/羟基磷灰石（PCL/HA）组织支架修复胸骨缺损。这些缺陷是在18只比格犬的胸骨肋关节上手术产生的。将大鼠分为空白组、PCL/HA组、PCL/HA/BMSCs组3组（n = 6）。影像学检查、组织学和组织形态计量学分析评估结果。空白组由于骨未愈合，缺损部位不能保持原有的完整性。在PCL/HA组和PCL/HA/BMSCs组中，观察到支架在12周时保持其形状，没有明显降解。两组均可通过x线片和组织学检查观察骨愈合情况。PCL/HA/BMSCs在种植部位矿化组织面积更大（p < 0.05）。结果表明，利用FDM技术制备具有特定凹槽的PCL/HA支架能较好地修复胸骨缺损。此外，骨髓间充质干细胞可促进骨长入，具有较好的应用前景。
The reconstruction of sternal defects remains clinically challenging for thoracic surgeons. Here we aimed to explore the individualized reconstruction of partial sternal defects with new biodegradable material in a large animal model. We used the fused deposition modeling (FDM) technique to manufacture polycaprolactone/hydroxyapatite (PCL/HA) tissue scaffolds with individualized grooves to repair the sternal defect. The defects were surgically created in a sternocostal joint of eighteen Beagle dogs. The animals were separated into three groups (n = 6): Blank group, PCL/HA group, and PCL/HA/BMSCs group. Radiographic examination, histological, and histomorphometric analyses were performed to evaluate the result. In the blank group, the defect site couldn't maintain its original integrity due to no bone union. In the PCL/HA group and PCL/HA/BMSCs group, it was observed that the scaffolds retained their shapes without significant degradation at 12 weeks. Both groups could observe new bone-union by radiographic and histological examination. And PCL/HA/BMSCs would be more mineralized tissue area at implant sites (p < 0.05). These results reveal that using the FDM technique to manufacture the PCL/HA scaffolds with specific grooves could repair the sternal defect satisfactorily. Furthermore the scaffolds with BMSCs-seeded could enhance the amount of bone ingrowth and seemed to be more promising.