Gene diversity and geographic differentiation in mitochondrial DNA of the Genji firefly, Luciola cruciata (Coleoptera: Lampyridae)
Gene diversity and geographic differentiation in mitochondrial DNA of the Genji firefly, Luciola cruciata (Coleoptera: Lampyridae)
复制标题
源氏萤 Luciola cruciata（鞘翅目：灯笼虫科）线粒体 DNA 的基因多样性和地理分化
DOI：
10.1006/mpev.2001.1046
复制
发表时间：
2002-02-01
影响因子：
4.1
通讯作者：
Ohba, N
中科院分区：
文献类型：
--
作者：
Suzuki, H;Sato, Y;Ohba, N
根据雌雄萤火虫求偶间隔的不同，将十字萤分为快闪型和慢闪型两种生态类型。为了评估这两种类型的进化起源，62个群体进行了线粒体细胞色素氧化酶（CO）11基因的限制性片段长度多态性分析。结果，共检测到19种单倍型，它们的分布是本地特有的。从单倍型序列比较构建的系统发育树显示三个主要分支（I，II和III）。分支I和分支11之间的单倍型边界与日本列岛的地质构造大致一致，这是一个巨大的断裂带，称为大窝，分支III和I-II的单倍型分布分别对应于九州和本州四国群岛。结果表明，在一个替代的情况下，目前L。cruciata多样性可能是由于日本列岛形成过程后的系统发育分离而产生的。CO 11基因树还表明，快闪型应该被认为是一种祖先形式，而慢闪型则是一种衍生形式。慢闪和快闪类型之间的分歧时间估计约为4.6至2.0百万年（上新世）。(C)2002 Elsevier Science（美国）。
The Genji firefly, Luciola cruciata, is divided into two ecological types, the fast-flash and slow-flash types, on the basis of the interflash interval of mate-seeking males. To evaluate the evolutionary origin of the two types, 62 populations were examined by restriction fragment length polymorphism analysis of the mitochondrial cytochrome oxidase (CO) 11 gene. As a result, 19 haplotypes were detected, and their distributions were indigenous to local areas. Phylogenetic trees constructed from sequence comparison of the haplotypes revealed three major clades (I, II, and III). The boundary of haplotypes between clades I and 11 is approximately concordant with the geological structure of the Japanese Islands, which is a great rupture zone called the Fossa Magna, and the distribution of haplotypes in clades III and I-II corresponds to the Kyushu and Honshu-Shikoku Islands, respectively. The results suggest a vicariant scenario in which current L. cruciata diversity would have arisen from phylogenetic separations subsequent to the formation process of the Japanese Islands based on the molecular clock. The CO 11 gene trees also suggested that the fast-flash type should be considered an ancestral form, while the slow-flash type would be a derived one. The divergence time between the slow- and the fast-flash types is estimated to be about 4.6 to 2.0 mya (the Pliocene epoch). (C) 2002 Elsevier Science (USA).