A mer operon confers mercury reduction in a Staphylococcus epidermidis strain isolated from Lanzhou reach of the Yellow River
A mer operon confers mercury reduction in a Staphylococcus epidermidis strain isolated from Lanzhou reach of the Yellow River
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DOI：
10.1016/j.ibiod.2014.02.002
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发表时间：
2014-05
影响因子：
4.8
通讯作者：
Zhengsheng Yu;Jing Li;Yabo Li;Qiang Wang;Xinping Zhai;Gaofeng Wu;Pu Liu;Xiangkai Li
中科院分区：
文献类型：
--
作者：
Zhengsheng Yu;Jing Li;Yabo Li;Qiang Wang;Xinping Zhai;Gaofeng Wu;Pu Liu;Xiangkai Li
兰州市汞(Hg)污染物普遍存在，造成环境污染。汞污染对环境和人类健康有害。微生物能够将汞(II)还原为毒性较低的元素汞，这可能是汞修复的潜在替代方案。我们从黄河兰州段的沉积物中分离到一株能够抵抗汞(II)的菌株Lzu-02。在含有40μM HgCl2的M9介质中，它也能够有氧地将汞(II)还原为元素汞。通过16S测序和VITEK表型鉴定，LZU-02菌株抵抗和还原汞(II)的最适条件为pH 6和37℃。我们还发现LZU-02含有由SmerR、SmerC、SmerT和SmerA基因组成的amer操纵子。当LZU-02在含40μM HgCl2的M9培养基中培养时，3个基因(SmerC、SmerT和SmerA)的相对表达上调。这些结果表明，菌株LZU-02可用于黄河兰州段汞(II)的生物修复。
Mercury (Hg) contaminants are widespread in Lanzhou and cause environmental pollution. Mercury pollution is harmful to environments and human health. Microbes are able to reduce Hg (II) to less toxic form elemental Hg which could be a potential alternative for mercury remediation. We've isolated a strain named Lzu-02 from sediments of the Lanzhou Reach of the Yellow River which is able to resist Hg (II). It was also able to reduce Hg (II) to elemental Hg aerobically when incubated in M9 medium with 40 μM HgCl2. The optimum condition of resisting and reducing Hg (II) for Lzu-02 was pH 6 and 37 °C. Lzu-02 was identified to be most similar toStaphylococcus epidermidisby 16S sequencing analysis and Vitek phenotype identification. We also found that Lzu-02 had ameroperon consisting ofSmerR,SmerC,SmerTandSmerAgenes. The relative expressions of three genes (SmerC,SmerTandSmerA) were upregulated when Lzu-02 was cultivated in M9 medium with 40 μM HgCl2. These results suggested that strain Lzu-02 could be useful for the bioremediation of Hg (II) in Lanzhou reach of the Yellow River.