Monoclonal antibodies that distinguish non-small cell from small cell lung cancer.
Monoclonal antibodies that distinguish non-small cell from small cell lung cancer.
复制标题
区分非小细胞肺癌和小细胞肺癌的单克隆抗体。
作者：
J. L. Mulshine;Frank Cuttitta;M. Bibro;J. Fedorko;S. Fargion;C. Little;D. Carney;A. Gazdar;J. Minna
用人大细胞肺癌细胞株(NCI-H157)免疫小鼠，制备了两株小鼠IgG2Ak单抗(703D4和704A1)，并进行了鉴定。这些抗体检测31kodalton[35S]蛋氨酸结合蛋白(S)上的不同表位。放射结合和免疫组织化学研究表明，这些抗体与大多数(11/13)人非小细胞肺癌(腺癌、表皮样癌和大细胞肺癌)结合，但不与小细胞肺癌(0/11)肿瘤结合。这些抗体识别的表位也在人类黑色素瘤(7/8)、另外两种肿瘤(骨肉瘤、肾细胞癌)上表达，但在许多其他人类肿瘤(乳腺、结肠、神经母细胞瘤、淋巴组织)上不表达，在一组正常成人组织上也不表达。由于抗原(S)在固定后被保存，而且由于它们能够区分肺癌类型和正常组织，它们应该具有临床和生物学价值。
Two murine IgG2Ak monoclonal antibodies (703D4, 704A 1) were produced and characterized after immunization with a human large cell lung cancer line (NCI-H 157). These antibodies detect different epitopes on 31 kilodalton [35S]methionine incorporating protein(s). Radiobinding and immunohistochemical studies show these antibodies bind to most (11/13) human non-small cell lung cancer (adenocarcinoma, epidermoid, and large cell), but not to small cell lung cancer (0/11) tumors tested. The epitopes these antibodies recognized are also expressed on human melanomas (7/8), two other tumors (osteogenic sarcoma, renal cell carcinoma), but not on many other human tumors (breast, colon, neuroblastoma, lymphoid), and not on a panel of normal adult human tissues. Because the antigen(s) are preserved after fixation and because of their ability to distinguish lung cancer types from each other and normal tissues, they should be of clinical, as well as of biologic interest.