DNA aneuploidy in early breast cancer.
DNA aneuploidy in early breast cancer.
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DOI：
10.1038/bjc.1995.421
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发表时间：
1995-10
影响因子：
8.8
通讯作者：
Andersen JA
中科院分区：
文献类型：
--
作者：
Ottesen GL;Christensen IJ;Larsen JK;Kerndrup GB;Hansen B;Andersen JA
对来自四组早期乳腺癌的148个未固定的冷冻组织样本进行高分辨率流式细胞术（FCM） DNA分析：以原位癌（DCIS）为主的浸润性癌（ICs） （I组）、临床小肿瘤<或= 15 mm （II组）、淋巴结阴性临床肿瘤（III组）和筛查检测的小肿瘤<或= 15 mm （IV组）。中位肿瘤大小为12mm。该研究的目的是通过更大的样本来支持我们最近在选择的DCIS优势ic组（I组）中关于DNA倍性模式的发现。在DNA非整倍体频率（79%和90%）、DNA指数（DI）分布、肿瘤内DNA异质性和s期分数方面，小型临床癌症和淋巴结阴性癌症的结果与该组相似。发现高频率的DNA超二倍体克隆，特别是与高度分化的肿瘤有关。与筛查检测到的癌症相比，发现了显著的差异，后者的特征是DNA非整倍体样本的频率要低得多（49%），可能代表了一组生物特异性的低恶性、缓慢生长的肿瘤。组织学分级与DI亚类之间、淋巴结状态与DNA二倍体/非整倍体之间存在关联，而DI与肿瘤大小无关。这一系列早期癌症的DNA倍性发现与我们自己从侵袭前病变以及从一系列更晚期癌症中报道的结果一致。
High-resolution flow cytometric (FCM) DNA analysis was performed on 148 unfixed, frozen tissue samples from four groups of early breast cancers: invasive carcinomas (ICs) with predominance of carcinoma in situ (DCIS) (group I), small clinical cancers < or = 15 mm (group II), node-negative, clinical cancers (group III) and small screening-detected cancers < or = 15 mm (group IV). The median tumour size was 12 mm. The aim of the study was to support, with a larger sample, our recent findings with respect to DNA ploidy pattern in the selected group of ICs with predominance of DCIS (group I). Similar results to this group were found for both the small clinical cancers and the node-negative cancers, with respect to frequency of DNA aneuploidy (79% and 90%), DNA index (DI) distribution, intratumoral DNA heterogeneity and S-phase fraction. A high frequency of DNA hyperdiploid clones was found, in particular related to highly differentiated tumours. A significant difference was found compared with the screening-detected cancers, which were characterised by a much lower frequency of DNA aneuploid samples (49%) and may represent a biologically specific group of low-malignant, slowly growing tumours. Associations were shown between histological grade and DI subclasses, and between lymph node status and DNA diploidy/aneuploidy, whereas DI was not correlated with tumour size. The DNA ploidy findings in this series of early cancers are concordant to our own results from preinvasive lesions as well as those reported from series of more advanced cancers.