The exposure to trans-cinnamic acid of osmotically stressed Catharanthus roseus cells cultured in a 14-1 bioreactor increases alkaloid accumulation
The exposure to trans-cinnamic acid of osmotically stressed Catharanthus roseus cells cultured in a 14-1 bioreactor increases alkaloid accumulation
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DOI：
10.1023/a:1005672400219
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发表时间：
2000-06-01
影响因子：
2.7
通讯作者：
Loyola-Vargas, VM
中科院分区：
文献类型：
--
作者：
Godoy-Hernández, GC;Vázquez-Flota, FA;Loyola-Vargas, VM
在14升生物反应器中培养长春花细胞系。当将该细胞系从 250 ml 批量培养物扩大到生物反应器时，总生物碱产量下降了 80% 以上。然而，随后施加渗透压和 1 mM 反式肉桂酸（抑制酚类化合物的合成），恢复了原来的生物碱含量。
A Catharanthus roseus cell line was cultured in a 14-l bioreactor. Total alkaloid production decreased more than 80% while scaling up this cell line from 250 ml batch cultures to the bioreactor. However, the subsequent application of an osmotic stress and 1 mM trans-cinnamic acid, which inhibits the synthesis of phenolic compounds, restored the original alkaloid amounts.