Accelerating microfluidic immunoassays on filter membranes by applying vacuum
Accelerating microfluidic immunoassays on filter membranes by applying vacuum
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DOI：
10.1007/s10544-011-9581-z
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发表时间：
2012-02
影响因子：
2.8
通讯作者：
Yingyi Liu;Jie Yu;Meihong Du;Wenjun Wang;Wei Zhang;Zhuo Wang;Xingyu Jiang
中科院分区：
文献类型：
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作者：
Yingyi Liu;Jie Yu;Meihong Du;Wenjun Wang;Wei Zhang;Zhuo Wang;Xingyu Jiang
本文介绍了一种真空加速微流控免疫分析技术（简称VAMI）。IgG的直接测定表明，VAMI可以同时实现更高的灵敏度，并需要更少的时间相比，传统的微流控免疫分析。进一步应用VAMI进行了3步竞争检测法（包括抗原固定化、竞争反应和第二抗体反应），用于检测非法食品添加剂苏丹红。总测定时间为15 min，检测限（LOD）为1 ng ml-1。
This paper describes a vacuum-accelerated microfluidic immunoassay (we abbreviate it as VAMI) by sandwiching a filter membrane between a two-layer chip. A direct assay of IgG demonstrated that VAMI could simultaneously achieve higher sensitivity and require less time compared with conventional microfluidic immunoassays. We further applied VAMI to carry out a 3-step competitive assay (including antigen immobilization, competitive reaction and 2ndantibody reaction) for detecting the illegal food additive Sudan Red. A total assay time of 15 min with a limit of detection (LOD) of 1 ng ml-1is achieved.