Characterization of an experimental miniature bioreactor for cellular perturbation studies
Characterization of an experimental miniature bioreactor for cellular perturbation studies
复制标题
DOI：
10.1002/bit.21003
复制
发表时间：
2006-12-20
影响因子：
3.8
通讯作者：
Heijnen, Joseph J.
中科院分区：
文献类型：
--
作者：
Aboka, Fredrick O.;Yang, Huiling;Heijnen, Joseph J.
微型生物反应器（3.0 mL体积）已被开发，并被证明是一种多功能工具，用于快速筛选和定量生物对环境扰动的反应。微型生物反应器本质上是一个塞流装置，通过肉汤的循环流动转化为一个混合良好的反应器。气相和液相由一层硅膜隔开。通过动态传质实验确定了氧和二氧化碳的传质能力。氧和二氧化碳的传质系数分别为1.55 +/- 0.17 x 10(-5) m/s和4.52 +/- 0.60 x 10(-6) m/s。3.0 mL生物反应器的培养实验表明：(1)它可以将生物量维持在与4.0 L实验室规模生物反应器相同的生理状态；(2)可以很容易地进行可重复的扰动实验，如改变底物吸收速率，并根据O-2和CO2的吸收和产生速率定量监测生理反应。(c) 2006 Wiley期刊公司
A mini bioreactor (3.0 mL volume) has been developed and shown to be a versatile tool for rapidly screening and quantifying the response of organisms on environmental perturbations. The mini bioreactor is essentially a plug flow device transformed into a well-mixed reactor by a recycle flow of the broth. The gas and liquid phases are separated by a silicone membrane. Dynamic mass transfer experiments were performed to determine the mass transfer capacities for oxygen and carbon dioxide. The mass transfer coefficients for oxygen and carbon dioxide were found to be 1.55 +/- 0.17 x 10(-5) m/s and 4.52 +/- 0.60 x 10(-6) m/s, respectively. Cultivation experiments with the 3.0 mL bioreactor show that (i) it can maintain biomass in the same physiological state as the 4.0 L lab scale bioreactor, (ii) reproducible perturbation experiments such as changing substrate uptake rate can be readily performed and the physiological response monitored quantitatively in terms of the O-2 and CO2 uptake and production rates. (c) 2006 Wiley Periodicals, Inc.