FIBROBLAST GROWTH-FACTOR IN THE EXTRACELLULAR-MATRIX OF DYSTROPHIC (MDX) MOUSE MUSCLE
FIBROBLAST GROWTH-FACTOR IN THE EXTRACELLULAR-MATRIX OF DYSTROPHIC (MDX) MOUSE MUSCLE
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DOI：
10.1126/science.2717945
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发表时间：
1989-05-12
期刊：
影响因子：
56.9
通讯作者：
STROHMAN, RC
中科院分区：
文献类型：
--
作者：
DIMARIO, J;BUFFINGER, N;STROHMAN, RC
多克隆抗体F547与牛碱性成纤维细胞生长因子和人重组碱性成纤维细胞生长因子反应，但不与牛酸性成纤维细胞生长因子反应。这种抗体定位于小鼠骨骼肌的细胞外基质中，主要是肌纤维内膜，其中包括含有肝素的基膜。在持续再生的MDX小鼠肌肉中，细胞外基质中的成纤维细胞生长因子水平高于对照组。MDX肌肉中基质成纤维细胞生长因子的过多可能与营养不良肌肉中卫星细胞和再生活性的增加有关，并可能有助于解释与基因相同的人类Duchenne肌营养不良症相比，MDX动物的良性表型。
Polyclonal antibody F547 reacts with a bovine basic fibroblast growth factor (bFGF) and a human recombinant bFGF, but not with bovine acidic fibroblast growth factor. This antibody localized bFGF in the extracellular matrix of mouse skeletal muscle, primarily in the fiber endomysium, which includes the heparin-containing basal lamina. In mdx mouse muscle, which displays persistent regeneration, FGF levels, in the extracellular matrix are higher than those in controls. Overabundance of matrix FGF in mdx muscles may be related to an increase in both satellite cell and regenerative activity in the dystrophic muscle and may help explain the benign phenotype of mdx animals compared with the genetically identical human Duchenne muscular dystrophy.