Phenotypic analysis for a cell line of canine epidermal keratinocytes
Phenotypic analysis for a cell line of canine epidermal keratinocytes
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DOI：
10.1292/jvms.70.853
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发表时间：
2008-08-01
影响因子：
1.2
通讯作者：
Fukata, Tsuneo
中科院分区：
文献类型：
--
作者：
Shibata, Sanae;Maeda, Sadatoshi;Fukata, Tsuneo
表皮角质形成细胞有可能产生炎症介质，这些介质被认为在特应性皮炎（AD）等皮肤疾病中发挥重要作用。因此，犬表皮角质形成细胞的细胞系可用于研究角质形成细胞的体外生物反应性。然而，目前还没有正确分析犬角质形成细胞系表型的报道。在这项工作中，我们对 CPEK 进行了表型分析。它是从成人木鞋的表皮中提取的，以检查与表皮角质形成细胞的表型相似性。目前的研究结果表明，CPEK 细胞表达表皮角质形成细胞的标志物，包括细胞角蛋白 14、α(6) 整合素和 PCNA。我们的研究结果表明，CPEK 可能是一种有用的细胞系，可用于研究表皮角质形成细胞在体外 AD 发病机制中的核心作用。
Epidermal keratinocytes have the potential to produce inflammatory mediators that are considered to play in important role in skin diseases such as atopic dermatitis (AD). Thus, cell lines of canine epidermal keratinocytes are useful for studying the biological reactivity of keratinocytes in vitro. However, there has been no report on properly analyzing the phenotype of canine keratinocyte cell lines. In this work, we performed phenotypic analysis of CPEK. which was derived from the epidermis of an adult clog in order to examine the phenotypic similarity with epidermal keratinocytes. The present findings indicated that CPEK cells expressed markers for epidermal keratinocytes including cytokeratin 14, alpha(6) integrin and PCNA. Our findings demonstrated that CPEK Could be a useful cell line for investigating the central role of epidermal keratinocytes in the pathogenesis of AD in vitro.