Analysis of B6 vitamers by micellar electrokinetic capillary chromatography with laser-excited fluorescence detection.
Analysis of B6 vitamers by micellar electrokinetic capillary chromatography with laser-excited fluorescence detection.
复制标题
采用胶束电动毛细管色谱法和激光激发荧光检测技术分析 B6 维生素B6 维生素A。
DOI：
10.1093/chromsci/24.8.347
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发表时间：
1986
影响因子：
1.3
通讯作者：
M. P. Maskarinec
中科院分区：
文献类型：
--
作者：
Burton De;M. Sepaniak;M. P. Maskarinec
胶束电动毛细管色谱 (MECC) 涉及在填充有胶束溶液的毛细管柱（内径 25 至 75 微米）上施加高压（10 至 40 kV）。这项工作中的流动相由磷酸盐/硼酸盐缓冲水溶液系统中的十二烷基硫酸钠组成。吡哆醇（维生素 B6）及其五种代谢物的分离效率高达 60,000 理论板数/米。使用激光激发荧光检测对人尿液中的维生素 B6 代谢物吡哆酸进行分离和定量。检测限小于注入的皮克。
Micellar electrokinetic capillary chromatography (MECC) involves the application of a high voltage (10 to 40 kV) across a capillary column (25 to 75 microns i.d.) that is filled with a solution containing micelles. The mobile phase in this work consists of sodium dodecyl sulfate in an aqueous phosphate/borate buffer system. Pyridoxine (vitamin B6) and five of its metabolites are separated, with efficiencies as high as 60,000 theoretical plates/meter. Pyridoxic acid, a metabolite of B6, is separated and quantitated in human urine using laser-excited fluorescence detection. Limits of detection are less than a picogram injected.