CD105 is a more appropriate marker for evaluating angiogenesis in urothelial cancer of the upper urinary tract than CD31 or CD34.
CD105 is a more appropriate marker for evaluating angiogenesis in urothelial cancer of the upper urinary tract than CD31 or CD34.
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DOI：
10.1007/s00428-013-1463-8
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发表时间：
2013-11
期刊：
影响因子：
3.5
通讯作者：
Sakai, Hideki
中科院分区：
文献类型：
--
作者：
Miyata, Yasuyoshi;Sagara, Yuji;Watanabe, Shin-ichi;Asai, Akihiro;Matsuo, Tomohiro;Ohba, Kojiro;Hayashi, Tomayoshi;Sakai, Hideki
血管生成在许多类型的癌症中的癌症进展中起重要作用。血管生成的评估经常进行，但人类癌症的最佳方法尚未达成一致。由于充分评估肿瘤组织中的血管生成可能对预测预后和治疗决策非常重要，因此我们通过评估上尿路尿路上皮癌（UC-UUT）中的微血管密度（MVD）来半定量评估血管生成。我们比较了三种内皮细胞标志物（CD 31、CD 34和CD 105）在122例确诊为UC-UUT且无转移的患者福尔马林固定组织上的表现。血管内皮生长因子（VEGF）-A的表达也进行了化学评价。研究了MVD与每种标记物和pT分期、分级、生存率和VEGF-A表达的相关性。抗CD 31、抗CD 34和抗CD 105免疫组化染色估计的平均（标准差）MVD分别为47.1（17.9）/高倍视野（HPF）、70.9（19.5）/HPF和31.2（16.7）/HPF。虽然所有MVD均与pT分期和分级显著相关，但CD 105-MVD显示出最强的相关性。CD 105-MVD与VEGF-A表达相关性最强（r = 0.530，p < 0.001）。尽管单因素分析显示所有MVD均与无转移生存率和病因特异性生存率相关，但在包括pT分期和分级的多因素分析中，仅CD 105-MVD被保留为独立预测因子。CD 105-MVD可能是半定量评估UC-UUT患者血管生成的首选标志物。
Angiogenesis plays an important role in cancer progression in many types of cancer. Evaluation of angiogenesis is often performed, but the optimal methodology for human cancer has not been agreed upon. As adequate evaluation of angiogenesis in cancer tissues might be important for prediction of prognosis and treatment decisions, we evaluated angiogenesis semiquantitatively by assessing microvessel density (MVD) in urothelial cancer of the upper urinary tract (UC-UUT). We compared the performance of three endothelial cell markers (CD31, CD34, and CD105) on formalin-fixed tissues from 122 patients diagnosed with UC-UUT without metastasis. Vascular endothelial growth factor (VEGF)-A expression was also evaluated immunohistochemically. Correlations between MVD with each marker and pT stage, grade, survival, and VEGF-A expression were investigated. Mean (standard deviation) MVD as estimated by immunohistochemical staining with anti-CD31, anti-CD34, and anti-CD105 were 47.1 (17.9)/high-power field (HPF), 70.9 (19.5)/HPF, and 31.2 (16.7)/HPF, respectively. Although all MVDs were significantly associated with pT stage and grade, CD105-MVD showed the strongest association. Similarly, CD105-MVD showed the strongest correlation with VEGF-A expression (r = 0.530, p < 0.001). Although all MVDs were associated with metastasis-free survival and cause-specific survival on univariate analysis, only CD105-MVD was retained as an independent predictor in multivariate analysis including pT stage and grade. CD105-MVD may be the preferred marker for semiquantitative assessment of angiogenesis in patients with UC-UUT.
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影响因子：
4.7
作者：
Rubatt, Jennifer M.;Darcy, Kathleen M.;Secord, Angeles Alvarez
通讯作者：
Secord, Angeles Alvarez
DOI：
10.1016/s1078-1439(03)00015-2
发表时间：
2004-01-01
影响因子：
2.7
作者：
Yang, CC;Chu, KC;Yeh, WM
通讯作者：
Yeh, WM
影响因子：
11.5
作者：
Miyata, Y;Kanda, S;Kanetake, H
通讯作者：
Kanetake, H
影响因子：
4.5
作者：
Friedrich, MG;Toma, MI;Huland, H
通讯作者：
Huland, H
影响因子：
6.6
作者：
Shariat, Shahrokh F.;Youssef, Ramy F.;Lotan, Yair
通讯作者：
Lotan, Yair