CYCLIC DIGUANYLIC ACID AND CELLULOSE SYNTHESIS IN AGROBACTERIUM-TUMEFACIENS
CYCLIC DIGUANYLIC ACID AND CELLULOSE SYNTHESIS IN AGROBACTERIUM-TUMEFACIENS
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DOI：
10.1128/jb.171.12.6649-6655.1989
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发表时间：
1989-12-01
影响因子：
3.2
通讯作者：
BENZIMAN, M
中科院分区：
文献类型：
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作者：
AMIKAM, D;BENZIMAN, M
研究了新型调节核苷酸双(3'',5'')-环状二鸟苷酸(c-di-GMP)的出现及其与植物病原体根癌农杆菌中纤维素生物合成的关系。在各种培养基中生长的 32P 标记细胞的酸提取物中检测到 c-di-GMP，并且发现在无细胞制剂中存在一种负责从 GTP 形成 c-di-GMP 的酶。使用[14C]葡萄糖作为示踪剂对体内纤维素合成进行定量评估。该生物体在没有植物细胞的情况下生长过程中产生纤维素，并且这种能力保留在静止细胞中。 c-di-GMP 及其前体 GTP 显着刺激了用膜制剂在体外从 UDP-葡萄糖合成纤维素产物，并进一步被 Ca2+ 增强。钙效应归因于对存在于含纤维素合酶的膜中的 c-di-GMP 降解酶的抑制。
The occurrence of the novel regulatory nucleotide bis(3'',5'')-cyclic diguanylic acid (c-di-GMP) and its relation to cellulose biogenesis in the plant pathogen Agrobacterium tumefaciens was studied. c-di-GMP was detected in acid extract of 32P-labeled cells grown in various media, and an enzyme responsible for its formation from GTP was found to be present in cell-free preparations. Cellulose synthesis in vivo was quantitatively assessed with [14C]glucose as a tracer. The organism produced cellulose during growth in the absence of plant cells, and this capacity was retained in resting cells. Synthesis of a cellulosic product from UDP-glucose in vitro with membrane preparations was markedly stimulated by c-di-GMP and its precursor GTP and was further enhanced by Ca2+. The calcium effect was attributed to inhibition of a c-di-GMP-degrading enzyme shown to be present in the cellulose synthase-containing membranes.