Assessment of genetic diversity and relationships among maize (Zea mays L.) Italian landraces by morphological traits and AFLP profiling
Assessment of genetic diversity and relationships among maize (Zea mays L.) Italian landraces by morphological traits and AFLP profiling
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DOI：
10.1007/s00122-008-0823-2
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发表时间：
2008-10-01
影响因子：
5.4
通讯作者：
Motto, M.
中科院分区：
文献类型：
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作者：
Hartings, H.;Berardo, N.;Motto, M.
在本研究中，我们利用形态特征和分子标记分析了 54 个意大利玉米地方品种样本的遗传多样性模式。尽管本研究调查的 54 个地方品种仅限于意大利玉米生产的核心地区伦巴第，但我们的数据显示所分析的种质在形态和分子性状方面存在很大的遗传异质性。此外，我们的数据证实 AFLP 标记产生高频率的多态性条带，并且能够明确地对所考虑的每个地方品种进行指纹识别。与形态学数据相比，基于 AFLP 标记的聚类分析显示了种质的更清晰的分离。不同的种群被分为四个主要集群，反映了所分析的地方品种的地理起源和季节性就业。分子方差分析显示组间、组内人群间以及人群内个体间存在显着差异（P < 0.01）。大约 74% 的总方差可归因于人群内部的差异。相反，各组之间的分化水平较低（接近 4%）。关于种群结构，种群之间的遗传距离（F-ST = 0.25 +/- 0.3）和群体内近交程度（F-SC = 0.22 +/- 0.2）没有显着差异，而两者与群体内标记之间的相关程度（F-CT = 0.04 +/- 0.03）存在显着差异。讨论了与合适的保存方法相关的结果。
In the present study we have analyzed the genetic diversity pattern in a sample of 54 Italian maize landraces, using morphological traits and molecular markers. Although the 54 landraces surveyed in this study were restricted to Lombardy, the core region of maize production in Italy, our data revealed a large genetic heterogeneity for both morphological and molecular traits in the accessions analyzed. Additionally, our data confirm that the AFLP markers produced a high frequency of polymorphic bands and were able to unequivocally fingerprint each of the landraces considered. Cluster analysis based on AFLP markers displayed a clearer separation of the accessions in comparison to morphological data. Different populations were divided into four major clusters reflecting the geographical origin and seasonal employment of the landraces analyzed. Molecular analysis of variance showed significant (P < 0.01) differences among groups, among populations within groups, and among individuals within populations. Approximately 74% of the total variance could be attributed to differences within populations. Conversely, a lower level of differentiation was detected among groups (similar to 4%). Regarding population structures, the genetic distance between populations (F-ST = 0.25 +/- 0.3) and the degree of inbreeding within groups (F-SC = 0.22 +/- 0.2), did not diverge significantly, while both significantly differed from the degree of relatedness between markers within groups (F-CT = 0.04 +/- 0.03). Results are discussed in relation to a suitable conservation method.