Trivalent Human Papillomavirus (HPV) VLP vaccine covering HPV type 58 can elicit high level of humoral immunity but also induce immune interference among component types.
Trivalent Human Papillomavirus (HPV) VLP vaccine covering HPV type 58 can elicit high level of humoral immunity but also induce immune interference among component types.
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DOI：
10.1016/j.vaccine.2010.02.057
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发表时间：
2010-04
期刊：
影响因子：
5.5
通讯作者：
Ting Zhang;Yu-fei Xu;L. Qiao;Youchun Wang;Xueling Wu;Dongsheng Fan;Qinglin Peng;Xue-mei Xu
中科院分区：
文献类型：
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作者：
Ting Zhang;Yu-fei Xu;L. Qiao;Youchun Wang;Xueling Wu;Dongsheng Fan;Qinglin Peng;Xue-mei Xu
人类乳头瘤病毒（HPV） 16/18型二价疫苗和16/18/6/11型四价疫苗已被证明是安全有效的，并获准供公众使用。然而，这两种疫苗并不完全符合中国、东南亚和拉丁美洲的HPV类型分布，这些地区的HPV 58高度流行。本研究在杆状病毒表达系统中制备了三种类型的病毒样颗粒（VLPs），制备了含有HPV 16、18和58 L1 VLPs的三价疫苗，并检测了其体外中和效价。该疫苗可诱导对各组分的高水平、长期体液免疫。但在比较三价疫苗与单价疫苗诱导的类型特异性中和抗体水平时，观察到免疫干扰。当将更多种类的VLPs配制成多价疫苗时，这种干扰会变得更加明显，但通过减少抗原剂量和添加适当的佐剂可以大大克服这种干扰。
Both Human Papillomavirus (HPV) type 16/18 bivalent vaccine and type 16/18/6/11 quadrivalent vaccine have been proved to be safe and effective, and licensed for public use. However, these two vaccines do not quite match the distribution of HPV types in China, Southeast Asia and Latin America, where HPV 58 is highly prevalent. Here we produced three types of virus-like particles (VLPs) in baculovirus expression system, formulated a trivalent vaccine containing HPV 16, 18, and 58 L1 VLPs and examined its in vitro neutralizing titers. This vaccine could induce high level and long-term humoral immunity against the component types. But immune interference was observed when comparing type specific neutralizing antibody levels induced by trivalent vaccine to those by corresponding monovalent vaccines. This kind of interference would become more obvious when formulating more types of VLPs into multivalent vaccines, but could be greatly overcome by decreasing the antigen dosage and adding a proper adjuvant.