High-throughput sheathless and three-dimensional microparticle focusing using a microchannel with arc-shaped groove arrays.
High-throughput sheathless and three-dimensional microparticle focusing using a microchannel with arc-shaped groove arrays.
复制标题
使用带有弧形凹槽阵列的微通道聚焦的高通量鞘和三维微粒。
DOI：
10.1038/srep41153
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发表时间：
2017-01-23
影响因子：
4.6
通讯作者：
Li W
中科院分区：
文献类型：
--
作者：
Zhao Q;Zhang J;Yan S;Yuan D;Du H;Alici G;Li W
利用二次流的高通量无鞘颗粒聚焦在微流体应用中具有很大的潜力。在这项工作中，提出了一种创新的粒子聚焦方法。这种方法利用了一种利用二次流和惯性迁移的机制。该装置是一个直通道，在顶面上具有弧形凹槽阵列。首先，通过流场数值模拟和力平衡分析，对聚焦机理和预期的聚焦现象进行了解释。以颗粒运动轨迹模拟为参考，设计了一系列实验，讨论了颗粒粒径、流量和沟槽结构数量变化对颗粒运动的影响。显微图像表明，该方法对不同粒径的颗粒均能很好地聚焦，且聚焦结果与理论和模拟结果吻合较好。最后，该通道成功地浓缩了Jurkat细胞，在生物检测领域显示了良好的兼容性。在这项工作中，弧形槽通道被证明有能力实现高通量，无鞘和三维粒子聚焦与简单的操作。
Sheathless particle focusing which utilises the secondary flow with a high throughput has great potential for use in microfluidic applications. In this work, an innovative particle focusing method was proposed. This method makes use of a mechanism that takes advantage of secondary flow and inertial migration. The device was a straight channel with arrays of arc-shaped grooves on the top surface. First, the mechanism and expected focusing phenomenon are explained using numerical simulation of the flow field and force balance. A simulation of particle trajectories was conducted as a reference, and then a series of experiments was designed and the effects of changes in particle size, flow rate and quantity of the groove structure were discussed. The microscopic images show that this particle focusing method performed well for different size particles, and the results agreed well with the theory and simulated results. Finally, the channel successfully concentrated Jurkat cells, which showed a good compatibility in the biological assay field. In this work, the arc-shaped groove channel was demonstrated to have the ability to achieve high-throughput, sheathless and three-dimensional particle focusing with simple operations.