Metal composition of ambient PM2.5 influences severity of allergic airways disease in mice.
Metal composition of ambient PM2.5 influences severity of allergic airways disease in mice.
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环境PM2.5的金属组成会影响小鼠过敏性气道疾病的严重程度。
DOI：
10.1289/ehp.6300
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发表时间：
2003-09
影响因子：
10.4
通讯作者：
Gilmour, M Ian
中科院分区：
文献类型：
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作者：
Gavett, Stephen H;Haykal-Coates, Najwa;Copeland, Lisa B;Heinrich, Joachim;Gilmour, M Ian
据报道，居住在德国东部Hettstedt的儿童对常见空气过敏原的致敏率高于居住在邻近城市泽布斯特的另一个队列;这些差异与工业空气污染的存在相关。1999年在Hettstedt采集的细颗粒物（空气动力学直径< 2.5微米; PM（2.5））样品中锌、镁、铅、铜和镉的含量比泽布斯特的样品高出几倍。为了确定流行病学研究的结果是否可以在动物模型中重复，我们将Hettstedt和泽布斯特的PM（2.5）给予卵清蛋白过敏小鼠。在Balb/c小鼠中，来自Hettstedt的PM（2.5），而不是来自泽布斯特或对照过滤器浸提液的PM（2.5），在激发前2小时抽吸时，引起对卵清蛋白激发的即时反应显著增加，但在致敏前2周抽吸时则没有。无论是在致敏前还是攻毒前给药，Hettstedt PM（2.5）均可增加抗原特异性IgE。气道对乙酰甲胆碱气雾剂的反应性和肺部炎症细胞数量仅在激发前暴露于Hettstedt PM（2.5）的过敏小鼠中显著增加。Hettstedt和泽布斯特PM（2.5）均显著增加肺损伤参数和促炎细胞因子。这些结果与流行病学调查结果一致，表明环境PM（2.5）的金属成分影响过敏性呼吸道疾病的严重程度。
Children living in Hettstedt in eastern Germany have been reported to have a higher prevalence of sensitization to common aeroallergens than another cohort living in the neighboring city of Zerbst; these differences correlated with the presence of industrial air pollution. Samples of fine particulate matter (< 2.5 micro m aerodynamic diameter; PM(2.5)) collected in Hettstedt in 1999 had several-fold higher levels of zinc, magnesium, lead, copper, and cadmium than samples from Zerbst. To determine if the results from epidemiologic studies could be repeated in an animal model, we administered PM(2.5) from Hettstedt and Zerbst to ovalbumin-allergic mice. In Balb/c mice, PM(2.5) from Hettstedt, but not PM(2.5) from Zerbst or control filter extract, caused a significant increase in immediate responses to ovalbumin challenge when aspirated 2 hr before challenge, but not when aspirated immediately before sensitization 2 weeks earlier. Antigen-specific IgE was increased by Hettstedt PM(2.5) whether administered before sensitization or challenge. Airway responsiveness to methacholine aerosol and lung inflammatory cell numbers were significantly increased only in allergic mice exposed to Hettstedt PM(2.5) before challenge. Both Hettstedt and Zerbst PM(2.5) significantly increased lung injury parameters and proinflammatory cytokines. These results are consistent with epidemiologic findings and show that metal composition of ambient PM(2.5) influences the severity of allergic respiratory disease.