Carrier Protein-mediated Transport of Neutral Amino Acids into Mung Bean Mitochondria.
Carrier Protein-mediated Transport of Neutral Amino Acids into Mung Bean Mitochondria.
复制标题
载体蛋白介导的中性氨基酸转运至绿豆线粒体。
DOI：
10.1104/pp.66.4.588
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发表时间：
1980
期刊：
影响因子：
7.4
通讯作者：
A. Huang
中科院分区：
文献类型：
--
作者：
A. Cavalieri;A. Huang
中性氨基酸向绿豆下胚轴线粒体的转运用溶胀法研究了其溶胀特性。分离的线粒体肿胀时，加入到等渗溶液的脯氨酸，丝氨酸，蛋氨酸，苏氨酸，丙氨酸和甘氨酸。溶胀是立体特异性的，因为它在l-氨基酸中比在相应的d-氨基酸中更快。预孵育的线粒体与巯基修饰试剂，对汞苯甲酸和汞，导致抑制的肿胀引起的脯氨酸，丝氨酸，苏氨酸和甘氨酸。丙氨酸引起的肿胀仅被汞撒利抑制，而蛋氨酸引起的肿胀仅被对苯甲酸汞抑制。在所有情况下，由巯基修饰试剂引起的抑制通过随后用二硫苏糖醇处理线粒体而容易逆转。N-乙基马来酰亚胺，另一种巯基改性剂，没有引起任何抑制的溶胀。研究结果表明，存在蛋白质介导的中性氨基酸进入植物线粒体的运输机制。
The transport of neutral amino acids into mitochondria isolated from the hypocotyl of mung bean (Roxb.) was studied by the swelling technique. Isolated mitochondria swelled when added to an isosmotic solution of proline, serine, methionine, threonine, alanine, and glycine. The swelling was stereospecific in that it was faster in the l-amino acid than in the corresponding d-amino acid. Preincubation of the mitochondria with the sulfhydryl modifying reagents, p-mercuribenzoate and mersalyl, resulted in an inhibition of the swelling caused by proline, serine, threonine, and glycine. The swelling induced by alanine was inhibited only by mersalyl, whereas that by methionine was inhibited only by p-mercuribenzoate. In all cases, the inhibition caused by the sulfhydryl modifying reagents was readily reversible by the subsequent treatment of the mitochondria with dithiothreitol. N-Ethylmaleimide, another sulfhydryl-modifying reagent, did not cause any inhibition of the swelling. The findings indicate the existence of a protein mediated mechanism for the transport of neutral amino acids into plant mitochondria.