PRIMARY STRUCTURE AND TRANSCRIPTION OF AN AMPLIFIED GENETIC-LOCUS - THE CUP1 LOCUS OF YEAST
PRIMARY STRUCTURE AND TRANSCRIPTION OF AN AMPLIFIED GENETIC-LOCUS - THE CUP1 LOCUS OF YEAST
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DOI：
10.1073/pnas.81.2.337
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发表时间：
1984-01-01
期刊：
影响因子：
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通讯作者：
FOGEL, S
中科院分区：
文献类型：
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作者：
KARIN, M;NAJARIAN, R;FOGEL, S
酵母的铜抗性是由CUP1位点控制的。抗性水平与该位点的拷贝数成正比，最多可在15个串联迭代拷贝中发现。为了阐明控制CUP1位点扩增和表达的分子机制，测定了其全核苷酸序列。对实验室酵母菌CUP1基本扩增单元内的两个转录单元进行了鉴定和定位。其中一个转录单位可被铜诱导并编码低分子量铜结合蛋白-铜螯合蛋白。由于基因扩增和转录诱导，螯合蛋白的产生增加，导致酵母细胞对Cu离子的抗性增加。
Cu resistance in yeast is controlled by the CUP1 locus. The level of resistance is proportional to the copy number of this locus, which can be found in up to 15 tandemly iterated copies. To elucidate the molecular mechanisms controlling the amplification and expression of the CUP1 locus, its full nucleotide sequence was determined. Two transcription units within the basic amplification unit of CUP1 in laboratory yeast strains were identified and mapped. One of those transcription units is inducible by Cu and encodes a low MW Cu binding protein-Cu chelatin. The increased production of chelatin, due to both gene amplification and induction of transcription, leads to increased resistance of yeast cells to Cu ions.