Dose Related Biodistribution & Elimination of 100% PFOB Emulsion
Dose Related Biodistribution & Elimination of 100% PFOB Emulsion
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剂量相关的生物分布
DOI：
10.3109/10731198809132639
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发表时间：
1988
影响因子：
2.4
通讯作者：
R. Mattrey
中科院分区：
文献类型：
--
作者：
R. Mitten;A. Burgan;A. Hamblin;G. Yee;D. Long;D. Long;R. Mattrey
全氟辛基溴(PFOB)已成功地作为氧载体和造影剂应用于X射线、计算机断层扫描、超声和核磁共振成像(MRI)。了解乳剂微粒摄取和消除的药代动力学知识对于确定合适的剂量和诊断性研究的时间至关重要。将180只大鼠分成0.3、0.6和1.0g/kg 3个剂量组，通过静脉注射100%PFOB乳剂来确定主要器官的生物分布、半衰期和全身半衰期。每组又分为6个亚组(n=10，S男，女5)。各亚组分别于注射后6或90分钟、1、2、7或28天处死。采集了16种不同的组织样品，用异辛烷萃取和气相色谱分析方法测定了PFOB的浓度。通过静脉注射PFOB测定全身半衰期。
Perfluoroctylbromide (PFOB) has been applied successfully as an oxygen carrier and a contrast agent for X-ray, computed tomography, ultrasound and Magnetic Resonance Imaging (MRI). Knowledge of the pharmacokinetics of emulsion particle uptake and elimination is critical for determining proper dose and timing of diagnostic studies. This study was intended to define major organ biodistribution and half-life as well as total body half-life as a function of dose.Determination of biodistribution and major organ half-life was achieved by administering 100% PFOB emulsion intravenously to 180 rats divided into 3 dose groups of 0.3, 0.6 & 1.0 g/kg. Each group was divided into 6 subgroups (n = 10; S male and 5 female). Each subgroup was sacrificed at either 6 or 90 min., 1,2,7, or 28 days post injection. 16 different tissue samples were collected and PFOB concentration determined by iso-octane extraction and gas chromatographic (GC) analysis. Total body half-life was determined by administering PFOB intravenously t...