lin-35/Rb and ubc-18, an E2 ubiquitin-conjugating enzyme, function redundantly to control pharyngeal morphogenesis in C-elegans
lin-35/Rb and ubc-18, an E2 ubiquitin-conjugating enzyme, function redundantly to control pharyngeal morphogenesis in C-elegans
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DOI：
10.1242/dev.00561
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发表时间：
2003-07-01
期刊：
影响因子：
4.6
通讯作者：
Darland, M
中科院分区：
文献类型：
--
作者：
Fay, DS;Large, E;Darland, M
视网膜母细胞瘤基因产物涉及多种细胞和发育过程的调节，包括在控制细胞周期进程中的明确作用。秀丽隐杆线虫视网膜母细胞瘤蛋白同源物LIN-35也是细胞周期进入的关键调节因子，并且如合成多外阴基因的研究所示，在外阴细胞命运的确定中起重要作用。我们证明了lin-35在器官形态发生中的额外和意想不到的功能。使用遗传学方法分离lin-35合成致死突变，我们已经确定了lin-35和ubc-18的冗余作用，ubc-18是一种编码与人UBCH 7密切相关的E2泛素结合酶的基因。LIN-35和UBC-18协同控制咽形态发生期间的一个或多个步骤。基于遗传和表型分析，lin-35在咽部形态发生中的作用似乎与其细胞周期相关功能不同。lin-35和ubc-18可以协同作用以调节C. elegans发育
The retinoblastoma gene product has been implicated in the regulation of multiple cellular and developmental processes, including a well-defined role in the control of cell cycle progression. The Caenorhabditis elegans retinoblastoma protein homolog, LIN-35, is also a key regulator of cell cycle entry and, as shown by studies of synthetic multivulval genes, plays an important role in the determination of vulval cell fates. We demonstrate an additional and unexpected function for lin-35 in organ morphogenesis. Using a genetic approach to isolate lin-35 synthetic-lethal mutations, we have identified redundant roles for lin-35 and ubc-18, a gene that encodes an E2 ubiquitin-conjugating enzyme closely related to human UBCH7. lin-35 and ubc-18 cooperate to control one or more steps during pharyngeal morphogenesis. Based on genetic and phenotypic analyses, this role for lin-35 in pharyngeal morphogenesis appears to be distinct from its cell cycle-related functions. lin-35 and ubc-18 may act in concert to regulate the levels of one or more critical targets during C. elegans development.