Essential fatty acids for larval mud crab Scylla serrata: implications of lack of the ability to bioconvert C18 unsaturated fatty acids to highly unsaturated fatty acids
Essential fatty acids for larval mud crab Scylla serrata: implications of lack of the ability to bioconvert C18 unsaturated fatty acids to highly unsaturated fatty acids
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DOI：
10.1016/s0044-8486(03)00542-8
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发表时间：
2004-03
期刊：
影响因子：
4.5
通讯作者：
M. Suprayudi;T. Takeuchi;K. Hamasaki
中科院分区：
文献类型：
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作者：
M. Suprayudi;T. Takeuchi;K. Hamasaki
研究了必需脂肪酸（EFA）对锯缘青蟹（Scylla serrata）幼体存活、发育和脂肪酸生物转化的影响。将泥蟹幼虫置于1升塑料烧杯（30 ind/l）中，在蚤状幼体（Z）1和Z2阶段喂食富含油酸（18：1 n −9，OA）、亚油酸（18：2n−6，LA）、亚麻酸（18：3 n −3，LNA）、二十碳五烯酸（20：5 n −3，EPA）和二十二碳六烯酸（22：6 n −3，DHA）乙酯油的轮虫。在达到Z3阶段时，轮虫被转移到未富集的卤虫或富集有EPA或DHA乙酯的卤虫。此外，为了比较富集轮虫对Z2期幼虫的脂肪酸组成的影响，在达到Z2期时收获喂食富集轮虫的幼虫用于脂肪酸分析。与轮虫相比，卤虫中所含的EFA对青蟹幼体的存活影响更大。饲喂未富集卤虫的泥蟹幼虫表现出EFA缺乏的迹象，例如存活率较低、蜕皮间隔期较长、第一蟹期甲壳宽度较窄，表明DHA上级EPA，其次是LNA和LA。Z2期幼虫摄食富含OA、LA和LNA的轮虫后，EPA和DHA的含量（g/100 g幼虫）分别从1.2下降到0.4-0.8和0.5下降到0.1-0.2。此外，单烯含量从1.4增加到2.5-3.2，表明膳食EFA缺乏。我们的结论是，青蟹幼虫有一个有限的或可以忽略不计的能力，将C18不饱和脂肪酸转化为高度不饱和脂肪酸。
This study was conducted to examine the effect of essential fatty acids (EFA) on the survival, development and bioconversion of fatty acids in mud crab Scylla serrata larvae. Mud crab larvae held in 1-l plastic beakers (30 ind/l) were fed rotifers that had been enriched with oleic acid (18:1n−9, OA), linoleic acid (18:2n−6, LA), linolenic acid (18:3n−3, LNA), eicosapentaenoic acid (20:5n−3, EPA) and docosahexaenoic acid (22:6n−3, DHA) ethyl ester oil during the zoea (Z)1 and Z2 stage. Upon reaching the Z3 stage, rotifers were shifted to unenriched Artemia or Artemia enriched with either EPA or DHA ethyl ester. Further in order to allow comparison of the effect of the enriched rotifers on the fatty acid composition of the Z2 stage larvae, larvae fed enriched rotifers were harvested upon reaching the Z2 stage for fatty acid analysis. The survival of mud crab larvae was more strongly affected by the EFA contained in the Artemia compared to rotifers. Mud crab larvae fed unenriched Artemia showed EFA deficiency signs such as lower survival, longer intermolt period, and a narrower carapace width at the first crab stage, indicating that DHA was superior to that of EPA and followed by LNA and LA. The fatty acid composition of the whole body of Z2 larvae fed rotifers enriched with OA, LA or LNA revealed that the content (g/100 g larvae) of EPA decreased from 1.2 to 0.4–0.8 and DHA decreased from 0.5 to 0.1–0.2, respectively. Moreover, the contents of monoenes increased from 1.4 to 2.5–3.2, indicating dietary EFA deficiency. We conclude that mud crab larvae have a limited or negligible capability to convert C18 unsaturated fatty acids to highly unsaturated fatty acids.