Biomineralization of an organophosphorus pesticide, Monocrotophos, by soil bacteria
Biomineralization of an organophosphorus pesticide, Monocrotophos, by soil bacteria
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DOI：
10.1046/j.1365-2672.2002.01680.x
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发表时间：
2002-01-01
影响因子：
4
通讯作者：
Kanekar, PP
中科院分区：
文献类型：
--
作者：
Bhadbhade, BJ;Sarnaik, SS;Kanekar, PP
目的：为研究久效磷（MCP）的生物矿化过程，并对MCP降解过程中的代谢产物进行鉴定，采用富集培养和适应培养技术，从暴露于MCP的土壤中分离得到2株细菌：黑蓝节杆菌（Arthrobacter atrocyaneus）MCM B-425和巨大芽孢杆菌（Bacillus megaterium）MCM B-423。在30 ℃摇瓶培养条件下，在含有1000 mg·l（-1）MCP的合成培养基中，两菌株在8 d内对MCP的降解率分别为93%和83%。培养物通过形成一种未知化合物-代谢物I、戊酸或乙酸和甲胺（作为中间代谢物），将MCP降解为二氧化碳、氨和磷酸盐。结论：黑蓝节杆菌MCM B-425和B-425是一种具有生物降解活性的微生物。megaterium MCM B-423能够将MCP矿化为二氧化碳、氨和磷酸盐。已经提出了涉及磷酸酶和酯酶的降解的微生物代谢途径。微生物培养物可用于久效磷污染废水或土壤的生物修复。
Aims: To study biomineralization of Monocrotophos (MCP) and identify the metabolites formed during biodegradation.Methods and Results: Two cultures, namely Arthrobacter atrocyaneus MCM B-425 and Bacillus megaterium MCM B-423, were isolated by enrichment and adaptation culture technique from soil exposed to MCP. The isolates were able to degrade MCP to the extent of 93% and 83%, respectively, from synthetic medium containing MCP at the concentration of 1000 mg l(-1) , within 8 d, under shake culture condition at 30 degreesC. The cultures degraded MCP to carbon dioxide, ammonia and phosphates through formation of one unknown compound - Metabolite I, valeric or acetic acid and methylamine, as intermediate metabolites. The enzymes phosphatase and esterase, reported to be involved in biodegradation of organophosphorus compounds, were detected in both the organisms.Conclusions: Arthrobacter atrocyaneus MCM B-425 and B. megaterium MCM B-423 isolated from soil exposed to MCP were able to mineralize MCP to carbon dioxide, ammonia and phosphates.Significance and Impact of the Study: Pathway for biodegradation of MCP in plants and animals has been reported. A microbial metabolic pathway of degradation involving phosphatase and esterase enzymes has been proposed. The microbial cultures could be used for bioremediation of wastewater or soil contaminated with Monocrotophos.