Fungicidal properties, sterol binding, and proteolytic resistance of the synthetic peptide D4E1
Fungicidal properties, sterol binding, and proteolytic resistance of the synthetic peptide D4E1
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DOI：
10.1139/cjm-44-6-514
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发表时间：
1998-06-01
影响因子：
2.8
通讯作者：
Walsh, TJ
中科院分区：
文献类型：
--
作者：
De Lucca, AJ;Bland, JM;Walsh, TJ
用黄曲霉、烟曲霉、尼日尔曲霉、串珠镰刀菌和尖孢镰刀菌的未萌发和萌发分生孢子研究了合成肽D4E1的杀菌特性。100%杀灭孢子萌发所需的最低致死浓度（MLC）。fumigatus、烟曲霉A. Flavus和A.尼日尔的浓度分别为12.5、12.5和25 μ M。两种镰刀菌分生孢子萌发和未萌发的MLC值。为3.0 μ M。除了A。D4E1对烟曲霉（Aspergillus spp.）物理化学研究表明，D4E1与麦角甾醇（一种存在于分生孢子壁中的甾醇）复合。胆固醇存在于F.串珠状藻对D4E1的亲和力大于麦角甾醇。D4E1对真菌和植物蛋白酶降解的抗性高于天然肽天蚕素A。这些体外结果表明D4E1是在植物中转基因表达以增强宿主对真菌感染的抗性的候选者。
The fungicidal properties of the synthetic peptide D4E1 were studied with nongerminated and germinating conidia of Aspergillus flavus, Aspergillus fumigatus, Aspergillus niger, Fusarium moniliforme, and Fusarium oxysporum. The minimal lethal concentrations (MLC) needed to kill 100% of germinating conidia of A. fumigatus, A. flavus, and A. niger were 12.5, 12.5, and 25 mu M, respectively. The MLC value for nongerminated and germinating conidia of both Fusarium spp. was 3.0 mu M. Except for A. fumigatus, D4E1 was inactive against the nongerminated conidia of the Aspergillus spp. Physicochemical studies showed D4E1 complexed with ergosterol, a sterol present in conidial walls. Cholesterol, present in nongerminated conidia of F. moniliforme, had a greater affinity for D4E1 than did ergosterol. D4E1 was more resistant to fungal and plant protease degradation than the natural peptide, cecropin A. These in vitro results suggest D4E1 is a candidate for transgenic expression in plants to enhance host resistance to fungal infection.