Specific and reversible immobilization of histidine-tagged proteins on functionalized silicon nanowires
Specific and reversible immobilization of histidine-tagged proteins on functionalized silicon nanowires
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DOI：
10.1088/0957-4484/21/24/245105
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发表时间：
2010-06-18
期刊：
影响因子：
3.5
通讯作者：
Martinez, Karen L.
中科院分区：
文献类型：
--
作者：
Liu, Yi-Chi C.;Rieben, Nathalie;Martinez, Karen L.
基于硅纳米线的场效应晶体管（FET）作为生物传感器（bioFET）在超灵敏和无标记检测生物分子相互作用方面显示出巨大的潜力。它们的灵敏度不仅取决于器件的性能，而且还取决于附着在硅纳米线上的生物识别基序的功能。在这项研究中，我们表明，硅纳米线可以化学官能化与Ni：NTA图案，适合于通过短的多组氨酸标签（His标签）在靠近硅纳米线表面的蛋白质的特定固定。我们证明，蛋白质保留其功能后，固定到SiNW。重要的是，在添加EDTA或咪唑后，Si NW上的蛋白质固定显示为可逆的，从而允许在需要时再生bioFET，例如在蛋白质具有有限寿命的情况下。我们预计，我们的方法可能会发现一个通用的bioFET开发利用功能蛋白质检测，因为它的高兼容性，各种类型的NW和蛋白质。
Silicon nanowire (Si NW)-based field effect transistors (FETs) have shown great potential as biosensors (bioFETs) for ultra-sensitive and label-free detection of biomolecular interactions. Their sensitivity depends not only on the device properties, but also on the function of the biological recognition motif attached to the Si NWs. In this study, we show that SiNWs can be chemically functionalized with Ni:NTA motifs, suitable for the specific immobilization of proteins via a short polyhistidine tag (His-tag) at close proximity to the SiNW surface. We demonstrate that the proteins preserve their function upon immobilization onto SiNWs. Importantly, the protein immobilization on the Si NWs is shown to be reversible after addition of EDTA or imidazole, thus allowing the regeneration of the bioFET when needed, such as in the case of proteins having a limited lifetime. We anticipate that our methodology may find a generic use for the development of bioFETs exploiting functional protein assays because of its high compatibility to various types of NWs and proteins.