Circulating microRNA Profiles in Patients with Type-1 Autoimmune Hepatitis.
Circulating microRNA Profiles in Patients with Type-1 Autoimmune Hepatitis.
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1型自身免疫性肝炎患者的循环microRNA谱。
DOI：
10.1371/journal.pone.0136908
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Yatsuhashi H
中科院分区：
文献类型：
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作者：
Migita K;Komori A;Kozuru H;Jiuchi Y;Nakamura M;Yasunami M;Furukawa H;Abiru S;Yamasaki K;Nagaoka S;Hashimoto S;Bekki S;Kamitsukasa H;Nakamura Y;Ohta H;Shimada M;Takahashi H;Mita E;Hijioka T;Yamashita H;Kouno H;Nakamuta M;Ario K;Muro T;Sakai H;Sugi K;Nishimura H;Yoshizawa K;Sato T;Naganuma A;Komatsu T;Oohara Y;Makita F;Tomizawa M;Yatsuhashi H
最近的研究表明，微（mi）RNA分子可以在循环中检测到，并可以作为各种疾病的潜在生物标志物。本研究使用微阵列分析来鉴定1型自身免疫性肝炎（AIH）患者与健康对照组相比异常表达的循环miRNA。从国家医院组织（NHO）-AIH-liver-network数据库中选择有充分记录且未经治疗的AIH患者。在接受治疗前，他们进行了血液采样和肝活检，并进行了炎症分级和纤维化分期。为了进一步证实微阵列数据，通过实时定量聚合酶链反应定量46例AIH患者、40例慢性丙型肝炎（CHC）患者和13例健康对照者的miR-21和miR-122循环表达水平。与微阵列数据一致，与CHC患者和健康对照相比，AIH患者的血清miR-21水平显著升高。血清miR-21和miR-122水平与丙氨酸氨基转移酶水平相关。miR-21和miR-122的循环水平在伴有肝硬化的AIH患者中显著降低，并且与纤维化分期的增加呈负相关。相比之下，循环miR-21水平与AIH的炎症组织学分级显著相关。我们推测，异常表达的血清miRNAs是AIH的潜在生物标志物，可能与AIH的发病机制有关。miR-21和miR-122血清水平的变化可以反映它们在AIH炎症过程中的介导作用。
Recent studies have demonstrated that micro (mi)RNA molecules can be detected in the circulation and can serve as potential biomarkers of various diseases. This study used microarray analysis to identify aberrantly expressed circulating miRNAs in patients with type 1 autoimmune hepatitis (AIH) compared with healthy controls. Patients with well-documented and untreated AIH were selected from the National Hospital Organization (NHO)-AIH-liver-network database. They underwent blood sampling and liver biopsy with inflammation grading and fibrosis staging before receiving treatment. To further confirm the microarray data, circulating expression levels of miR-21 and miR-122 were quantified by real-time quantitative polymerase chain reaction in 46 AIH patients, 40 patients with chronic hepatitis C (CHC), and 13 healthy controls. Consistent with the microarray data, serum levels of miR-21 were significantly elevated in AIH patients compared with CHC patients and healthy controls. miR-21 and miR-122 serum levels correlated with alanine aminotransferase levels. Circulating levels of miR-21 and miR-122 were significantly reduced in AIH patients with liver cirrhosis, and were inversely correlated with increased stages of fibrosis. By contrast, levels of circulating miR-21 showed a significant correlation with the histological grades of inflammation in AIH. We postulate that aberrantly expressed serum miRNAs are potential biomarkers of AIH and could be implicated in AIH pathogenesis. Alternations of miR-21 and miR-122 serum levels could reflect their putative roles in the mediation of inflammatory processes in AIH.