A recent outbreak of human immunodeficiency virus type 1 infection in southern China was initiated by two highly homogeneous, geographically separated strains, circulating recombinant form AE and a novel BC recombinant
A recent outbreak of human immunodeficiency virus type 1 infection in southern China was initiated by two highly homogeneous, geographically separated strains, circulating recombinant form AE and a novel BC recombinant
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DOI：
10.1128/jvi.74.23.11286-11295.2000
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发表时间：
2000-12-01
影响因子：
5.4
通讯作者：
Yu, XF
中科院分区：
文献类型：
--
作者：
Piyasirisilp, S;McCutchan, FE;Yu, XF
在中国，注射吸毒者（IDUs）中人类免疫缺陷病毒I型（HIV - 1）的新爆发正沿着海洛因贩运路线蔓延。近期，中国南方广西报告了两起分别在注射吸毒者中发生的HIV - 1流行事件，对env基因的部分测序显示为C亚型和流行重组形式（CRF）AE。我们评估了来自广西注射吸毒者的5个近乎全长的HIV - 1基因组序列，以确定基因多样性以及亚型间重组体的存在情况。序列分析显示出两个在地理上分离的、高度同源的HIV - 1毒株。在靠近滇桂边界山区的百色市的3名注射吸毒者中发现了B/C亚型间重组体。这些重组体大多为C亚型，其衣壳和逆转录酶（RT）基因的部分来自B亚型。衣壳的B亚型部分位于N -末端结构域，该结构域已被证明会影响病毒核心成熟、病毒感染性以及与亲环蛋白A的结合，而RT的B亚型部分位于掌状亚结构域，这是该酶的活性位点。这些BC重组体不同于在中国西北新疆省发现的一种BC重组体。在广西首府南宁的注射吸毒者以及中越边境附近凭祥市的注射吸毒者中发现了CRF AE毒株。A - E和BC重组体均因其患者间低多样性而引人注目，全基因组的差异小于1%。HIV - 1在注射吸毒者中的快速传播可能促使高度同源毒株的出现，包括在具有多种亚型的地区出现新型重组体。
New outbreaks of human immunodeficiency virus type I (HIV-1) among injecting drug users (IDUs) are spreading in China along heroin trafficking routes. Recently, two separate HIV-1 epidemics among IDUs were reported in Guangxi, Southern China, where partial sequencing of the env gene showed subtype C and circulating recombinant form (CRF) AE. We evaluated five virtually full-length HIV-1 genome sequences from IDUs in Guangxi to determine the genetic diversity and the presence of intersubtype recombinants. Sequence analysis showed two geographically separated, highly homogeneous HIV-1 strains. B/C intersubtype recombinants were found in three IDUs from Baise City, in a mountainous region near the Yunnan-Guangxi border. These were mostly subtype C, with portions of the capsid and reverse transcriptase (RT) genes from subtype B. The subtype B portion of the capsid was located in the N-terminal domain, which has been shown to influence virus core maturation, virus infectivity, and binding to cyclophilin A, whereas the subtype B portion of RT was located in the palm subdomain, which is the active site of the enzyme. These BC recombinants differed from a BC recombinant found in Xinjiang Province in northwestern China. CRF AE strains were found in IDUs from Nanning, the capital of Guangxi, and in IDUs from Pingxiang City near the China-Vietnam border. The A-E and BC recombinants were both remarkable for their low interpatient diversity, less than 1% for the full genome. Rapid spread of HIV-1 among IDUs may foster the emergence of highly homogeneous strains, including novel recombinants in regions with multiple subtypes.