A wearable graphene transistor-based biosensor for monitoring IL-6 biomarker
A wearable graphene transistor-based biosensor for monitoring IL-6 biomarker
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DOI：
10.1016/j.mee.2022.111835
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发表时间：
2022-06-01
影响因子：
2.3
通讯作者：
Song, Edward
中科院分区：
文献类型：
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作者：
Laliberte, Kaitlyn E.;Scott, Patrick;Song, Edward
基于石墨烯的场效应晶体管（GFET）正在成为一种越来越流行的生物传感平台，用于通过生物标志物检测来监测健康状况。此外，石墨烯的二维几何形状使其成为实现柔性或可穿戴电子设备的理想选择。如果作为可穿戴生物传感器实现，此类技术可以无创地实时连续监测相关生物标志物，并提醒用户可能的健康问题。作为可行性证明，本文提出了一种在柔性薄膜上制造的可穿戴 GFET 设备，能够检测浓度范围为 10 pM 至 100 nM 的白细胞介素 6 (IL-6) 蛋白，这是一种与免疫反应有关的关键生物标志物。石墨烯的表面用靶标结合适体进行修饰，以确保分析物的选择性。我们的结果表明，当 GFET 以 1.5 cm 至 4.25 cm 之间的曲率半径弯曲时，生物传感器测量结果稳定且变化最小，这表明柔性 GFET 器件具有鲁棒性。我们还展示了在 10 pM 和 1 nM 浓度范围内以高灵敏度连续实时监测 IL6。此外，还开发了占用空间最小的电池供电电路板，用于控制 GFET 并实时记录传感器响应，证明成为完全独立的可穿戴生物传感器的可行性。这项工作的结果表明，基于 GFET 的薄膜生物传感器有潜力用作可穿戴连续健康监测设备。
Graphene-based field-effect transistor (GFET) is becoming an increasingly popular biosensing platform for monitoring health conditions through biomarker detection. Moreover, the graphene's 2-dimensional geometry makes it ideal for implementing flexible or wearable electronic devices. If implemented as a wearable biosensor, such technology can non-invasively monitor relevant biomarkers continuously in real-time and alert the user of possible health concerns. As a proof of feasibility, this paper presents a wearable GFET device fabricated on a flexible film that is capable of detecting interleukin-6 (IL-6) protein, a key biomarker implicated in immune responses, in the concentration range of 10 pM to 100 nM. The surface of graphene is modified with targetbinding aptamers to ensure analyte selectivity. Our results show that the biosensor measurements were stable with minimum changes when the GFET was bent with a radius of curvature between 1.5 cm and 4.25 cm suggesting robustness of the flexible GFET device. We have also demonstrated continuous real-time monitoring of IL6 with high sensitivity within the concentration range of 10 pM and 1 nM. Furthermore, a minimum footprint, battery-powered circuit board is also developed that controls the GFET and records the sensor responses in realtime demonstrating the feasibility of becoming a fully standalone and wearable biosensor. The results from this work suggest that the thin film GFET-based biosensor has the potential to be used as a wearable continuous health monitoring device.