Aspergillus-induced superoxide production by cystic fibrosis phagocytes is associated with disease severity
Aspergillus-induced superoxide production by cystic fibrosis phagocytes is associated with disease severity
复制标题
DOI：
10.1183/23120541.00068-2017
复制
发表时间：
2018-04-01
影响因子：
4.6
通讯作者：
Warris, Adilia
中科院分区：
文献类型：
--
作者：
Brunel, Shan F.;Willment, Janet A.;Warris, Adilia
烟曲霉感染高达50%的囊性纤维化（CF）患者，并可能在进行性肺部疾病中发挥作用。由于囊性纤维化跨膜传导调节因子在先天免疫系统的细胞中表达，我们假设受损的抗真菌免疫应答在CF相关的Aspergillus lung disease.外周血单核细胞，多形核细胞（PMN）和单核细胞从CF患者和健康志愿者的血液样品中分离。活细胞成像和比色测定用于评估体外抗真菌活性。使用鲁米诺诱导的化学发光来测量活性氧物质（ROS）的产生，并且与通过病例报告表收集的临床指标相关。烟曲霉分生孢子CF吞噬细胞产生的ROS比健康对照组高4倍（p
Aspergillus fumigatus infects up to 50% of cystic fibrosis (CF) patients and may play a role in progressive lung disease. As cystic fibrosis transmembrane conductance regulator is expressed in cells of the innate immune system, we hypothesised that impaired antifungal immune responses play a role in CF-related Aspergillus lung disease.Peripheral blood mononuclear cells, polymorphonuclear cells (PMN) and monocytes were isolated from blood samples taken from CF patients and healthy volunteers. Live-cell imaging and colorimetric assays were used to assess antifungal activity in vitro. Production of reactive oxygen species (ROS) was measured using luminol-induced chemiluminescence and was related to clinical metrics as collected by case report forms.CF phagocytes are as effective as those from healthy controls with regards to phagocytosis, killing and restricting germination of A. fumigatus conidia. ROS production by CF phagocytes was up to four-fold greater than healthy controls (p