Integrated physiological and transcriptomic analyses of two warm- and cool-season turfgrass species in response to heat stress
Integrated physiological and transcriptomic analyses of two warm- and cool-season turfgrass species in response to heat stress
复制标题
两种暖季型和冷季型草坪草对热应激的综合生理和转录组分析
DOI：
10.1016/j.plaphy.2021.12.013
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发表时间：
2022
影响因子：
6.5
通讯作者：
Zhulong Chan
中科院分区：
文献类型：
--
作者：
Mengyao Liu;Tianxiao Sun;Chunling Liu;Hui Zhang;Weiliang Wang;Yanping Wang;Lin Xiang;Zhulong Chan
Warm- and cool-season turfgrasses were originated from different locations with contrasting heat tolerance. The molecular mechanisms of heat tolerance have not been extensively studied in turfgrass species. In this study, transcriptomic analysis showed that bermudagrass was more tolerant to heat stress as evidenced by lower contents of H2O2, proline and glutathione than those in tall fescue after heat treatment. RNA sequencing analysis revealed that 32.7% and 17.7% more genes were changed in tall fescue than in bermudagrass after 2 and 12h heat treatment, respectively. GO terms of redox were enriched in bermudagrass whereas metabolite transportation ones were over-represented in tall fescue after 2h treatment. Ubiquitin dependent degradation pathways were commonly regulated in both grass species.CdF-boxandFaF-boxtransgenic Arabidopsis exhibited improved tolerance to heat stress. Regulatory elements analysis revealed that four ABA-responsive elements present inCdF-boxpromoter, indicatingCdF-boxcould be potentially regulated by ABRE binding factors (ABFs). All these findings provide evidences for understanding heat stress response in warm- and cool-season grass species.
