Biocompatible Lipid-Coated Persistent Luminescent Nanoparticles for In Vivo Imaging of Dendritic Cell Migration
Biocompatible Lipid-Coated Persistent Luminescent Nanoparticles for In Vivo Imaging of Dendritic Cell Migration
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DOI：
10.1002/ppsc.201900371
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发表时间：
2019-09-27
影响因子：
2.7
通讯作者：
Braeckmans, Kevin
中科院分区：
文献类型：
--
作者：
Harizaj, Aranit;De Clercq, Olivier Q.;Braeckmans, Kevin
在过去十年中，基于树突状细胞（DC）的免疫治疗疫苗已经取得了可喜的成果。为了进一步改进目前的治疗方案并提高治疗效果，无创的体内DCs跟踪仍然至关重要。持久性发光纳米粒子（PLNPs）是一种无机材料，在光激发停止后数小时仍能显示出余辉。如果余辉是近红外的，则可以在体内跟踪注射颗粒的发射。然而，稳定性和毒性问题限制了裸PLNPs在生物学应用中的使用。因此，需要适当的表面功能化来提高其生物相容性。在这项研究中，研究人员证明了近红外光发光的LiGa5O8:Cr3+纳米粒子可以用生物相容性脂质涂层进行功能化，从而使其在生物介质中具有出色的稳定性。体外实验表明，即使在非常高的颗粒浓度下，也没有细胞毒性，并且对树突状细胞的成熟潜力没有不利影响。用脂质包被的LiGa5O8:Cr3+纳米颗粒标记的树突细胞注射到小鼠体内，可以在数天内成像，证实其在体内有效地迁移到腘窝淋巴结。总之，这些结果表明，脂质包被的LiGa5O8:Cr3+纳米颗粒在研究和开发基于DC的疫苗方面具有良好的可能性。
Dendritic cell (DC)-based vaccines for immunotherapy have already achieved promising results in the last decade. To further improve current treatment protocols and enhance the therapeutic outcome, noninvasive in vivo tracking of DCs remains of crucial importance. Persistent luminescent nanoparticles (PLNPs) are inorganic materials which show an afterglow for hours after the optical excitation has ceased. If the afterglow is in the near-infrared, the emission of injected particles can be tracked in vivo. However, stability and toxicity issues limit the use of bare PLNPs for biological applications. Therefore, appropriate surface functionalization is needed to improve their biocompatibility. In this study, it is demonstrated that near-infrared light emitting LiGa5O8:Cr3+ nanoparticles can be functionalized with a biocompatible lipid coating which provides them with outstanding stability in biological media. In vitro experiments show efficient uptake, absence of cytotoxicity even at very high particle concentrations, and no adverse effects on the maturation potential of DCs. DCs labeled with lipid-coated LiGa5O8:Cr3+ nanoparticles injected in mice can be imaged over days, confirming efficient in vivo migration to the popliteal lymph node. Together the results show that lipid coated LiGa5O8:Cr3+ nanoparticles possess excellent possibilities for further use in research and development of DC based vaccines.