Monocyte-macrophage modulation of T-lymphocyte-derived colony-stimulating activity elaboration in man.
Monocyte-macrophage modulation of T-lymphocyte-derived colony-stimulating activity elaboration in man.
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单核细胞-巨噬细胞对人类 T 淋巴细胞衍生集落刺激活性的调节。
DOI：
10.1111/j.1600-0609.1982.tb00524.x
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
McCredie,KB
中科院分区：
文献类型：
--
作者：
Verma,DS;Spitzer,G;Johnston,DA;Beran,M;Zander,AR;McCredie,KB
To investigate the source and the mechanisms of synergistically enhanced colony‐stimulating activity elaboration by the coincubated monocyte‐macrophages and T lymphocytes, we simultaneously prepared conditioned media both from the coincubated monocyte‐macrophages and T lymphocytes (ratio 1:3) in the presence of phytohemagglutinin (1%) or methanol extraction residue of bacillus Calmette‐Guerin (50 μg/ml) and from the isolated T lymphocytes that had been primed with monocyte‐macrophages in the presence or absence of phytohemagglutinin or methanol extraction residue of bacillus Calmette‐Guerin. Subsequently, colony‐stimulating activity in various conditioned media was assayed using light‐density (< 1.070 g/ml), nonadherent normal human marrow cells. Live monocyte‐macrophages synergized with and significantly (P < 0.01) agumented the T lymphocyte‐derived colony‐stimulating activity elaboration; while killed monocytes‐macrophages had no such effect. Similarly, actinomycin D and cycloheximide not only diminished monocyte‐macrophage colony‐stimulating activity elaboration but also reduced their synergistic interaction with T lymphocytes and their ability to augment the T lymphocyte‐derived colony‐stimulating activity elaboration. In contrast, mitomycin C failed to diminish both ‐ monocyte‐macrophages' ability to synergise with T lymphocytes and also to augment T lymphocyte‐derived colony‐stimulating activity. These data suggest that monocyte‐macrophages require an intact transcriptional and translational processes, but not DNA synthesis for synergising with T lymphocytes or for augmenting T lymphocyte colony‐stimulating activity elaboration.
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在有丝分裂原刺激的淋巴细胞培养物中产生集落刺激因子。
影响因子：
4.4
作者：
John W. Parker;Donald Metcalf
通讯作者：
Donald Metcalf
T 淋巴细胞和单核细胞-巨噬细胞在人类集落刺激活性阐述中的相互作用。
影响因子：
20.3
作者：
D. Verma;G. Spitzer;A. Zander;R. Fisher;K. McCredie;K. Dicke
通讯作者：
K. Dicke
调节有丝分裂原刺激的淋巴细胞释放集落刺激活性。
影响因子：
4.4
作者：
F. Ruscetti;P. Chervenick
通讯作者：
P. Chervenick
人外周血白细胞抗原诱导的胚芽生成反应中巨噬细胞-淋巴细胞的相互作用。
影响因子：
4.4
作者：
Evan M. Hersh;Jules E. Harris
通讯作者：
Jules E. Harris
人血单核细胞：体外粒细胞和单核集落形成的刺激剂
影响因子：
56.9
作者：
P. Chervenick;A. Lobuglio
通讯作者：
A. Lobuglio