TLC1 - TEMPLATE RNA COMPONENT OF SACCHAROMYCES-CEREVISIAE TELOMERASE
TLC1 - TEMPLATE RNA COMPONENT OF SACCHAROMYCES-CEREVISIAE TELOMERASE
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DOI：
10.1126/science.7545955
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发表时间：
1994-10-21
期刊：
影响因子：
56.9
通讯作者：
GOTTSCHLING, DE
中科院分区：
文献类型：
--
作者：
SINGER, MS;GOTTSCHLING, DE
端粒是真核生物线性染色体的自然末端，对染色体的稳定性至关重要。由于DNA复制的本质，端粒需要一种特殊的机制来确保其完全复制。端粒还能够沉默位于端粒附近的基因的转录。为了确定在萌芽酵母中对端粒功能起重要作用的基因，我们进行了一项筛选，寻找当大量表达时会抑制端粒沉默的基因。这一筛选导致了TLC1(端粒酶组分1)基因的鉴定。TLC1编码端粒酶的模板RNA，端粒酶是一种在各种生物体中复制端粒所需的核糖核蛋白。TLC1的发现证实了酿酒酵母中端粒酶的存在，并可能有助于对该酶的分析和对端粒结构和功能的理解。
Telomeres, the natural ends of linear eukaryotic chromosomes, are essential for chromosome stability. Because oi the nature of DNA replication, telomeres require a specialized mechanism to ensure their complete duplication. Telomeres are also capable of silencing the transcription of genes that are located near them. In order to identify genes in the budding yeast Saccharomyces cerevisiae that are important for telomere function, a screen was conducted for genes that, when expressed in high amounts, would suppress telomeric silencing. This screen lead to the identification of the gene TLC1 (telomerase component 1). TLC1 encodes the template RNA of telomerase, a ribonucleoprotein required for telomere replication in a variety of organisms. The discovery of TLC1 confirms the existence of telomerase in S. cerevisiae and may facilitate both the analysis of this enzyme and an understanding of telomere structure and function.