Selective inhibition of membrane ATPases by hydrogen peroxide in the lens of the eye.
Selective inhibition of membrane ATPases by hydrogen peroxide in the lens of the eye.
复制标题
眼睛晶状体中过氧化氢选择性抑制膜 ATP 酶。
DOI：
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复制
发表时间：
1988
期刊：
影响因子：
--
通讯作者：
N. Delamere
中科院分区：
文献类型：
--
作者：
D. Borchman;C. Paterson;N. Delamere
Oxidative damage to the lens resulting in decreased Ca-ATPase activity may play a significant role in the formation of cataracts. Deterioration of membrane function is implicated in 70% of all human lens cataracts since the intracellular calcium and sodium content of these lenses are dramatically elevated.1 Since oxidative damage to ion transport mechanisms might contribute to cataract formation,2 we chose to study the effect of hydrogen peroxide on Na-K-ATPase and Ca-ATPase activity. In fact, significant concentrations of hydrogen peroxide has been reported in the vicinity of the lens in both the human and rabbit eye in vivo. We prepared a membrane-enriched sample from Lens epithelium, cortex, and nucleus. The preparation is the same as that used routinely for preparing cardiac membranes enriched with Ca-ATPase.3 This procedure was used because commonly used lens membrane preparations utilize chaotropic agents to solubilize cytosolic lens crystallins. These chaotropic agents abolish Ca-ATPase activity. Gel electrophoresis, lipid and protein quantitation, and ATPase activity show that our preparation is enriched with plasma membranes. The lipid:protein ratio was increased 22- and 23- fold in our final cortical and nuclear membrane preparations, respectively. Total phospholipid recovery was 98.6%.
离体心脏肌浆网中钙调蛋白介导的钙转运调节和 (Ca2 Mg2 ) 激活的 ATP 酶活性。
DOI：
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发表时间：
1982
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Kirchberger,MA;Antonetz,T
通讯作者：
Antonetz,T
Na-K ATP酶在青蛙和牛晶状体中的分布。
影响因子：
2
作者：
Alvarez,LJ;Candia,OA;Grillone,LR
通讯作者：
Grillone,LR