Utilizing the planarian voltage-gated ion channel transcriptome to resolve a role for a Ca(2+) channel in neuromuscular function and regeneration.
Utilizing the planarian voltage-gated ion channel transcriptome to resolve a role for a Ca(2+) channel in neuromuscular function and regeneration.
复制标题
DOI：
10.1016/j.bbamcr.2016.10.010
复制
发表时间：
2017-06
期刊：
影响因子：
--
通讯作者：
Marchant JS
中科院分区：
文献类型：
--
作者：
Chan JD;Zhang D;Liu X;Zarowiecki M;Berriman M;Marchant JS
涡虫的强大再生能力依赖于信号事件的协调，从早期损伤反应到干细胞制定的分化结果，恢复适当的组织类型。可兴奋细胞中的急性信号事件在决定再生极性中起着重要作用，这一发现被表皮下肌肉细胞表达已知控制再生结果的关键模式基因所合理化。这些数据表明Ca2+信号在涡虫再生中的双重传导（神经肌肉信号）和指导（前后模式）作用。在这里，为了便于研究可兴奋细胞生态位中的急性信号事件，我们提供了一个来自涡虫Dugesia japonica的从头转录组组装，允许表征这种模式生物的多种嗜离子性组合。我们通过进一步描述每个涡虫在再生过程中电压操作的Ca2+通道的个体作用来证明这一资源的实用性，并证明了特定电压操作的Ca2+通道（Cav1B）的敲低会损害肌肉功能，从而独特地创造一个有利于前化的环境。提供完整的转录组数据集将有助于进一步研究涡虫电压门控通道组合中的分子，以探索可兴奋细胞生理学在再生结果中的作用。
The robust regenerative capacity of planarian flatworms depends on the orchestration of signaling events from early wounding responses through the stem cell enacted differentiative outcomes that restore appropriate tissue types. Acute signaling events in excitable cells play an important role in determining regenerative polarity, rationalized by the discovery that sub-epidermal muscle cells express critical patterning genes known to control regenerative outcomes. These data imply a dual conductive (neuromuscular signaling) and instructive (anterior-posterior patterning) role for Ca2+ signaling in planarian regeneration. Here, to facilitate study of acute signaling events in the excitable cell niche, we provide a de novo transcriptome assembly from the planarian Dugesia japonica allowing characterization of the diverse ionotropic portfolio of this model organism. We demonstrate the utility of this resource by proceeding to characterize the individual role of each of the planarian voltage-operated Ca2+ channels during regeneration, and demonstrate that knockdown of a specific voltage operated Ca2+ channel (Cav1B) that impairs muscle function uniquely creates an environment permissive for anteriorization. Provision of the full transcriptomic dataset should facilitate further investigations of molecules within the planarian voltage-gated channel portfolio to explore the role of excitable cell physiology on regenerative outcomes.