CELL-FREE TRANSLATION OF SOIL-BORNE WHEAT MOSAIC-VIRUS RNAS
CELL-FREE TRANSLATION OF SOIL-BORNE WHEAT MOSAIC-VIRUS RNAS
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DOI：
10.1016/0042-6822(85)90114-x
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发表时间：
1985-01-01
期刊：
影响因子：
3.7
通讯作者：
BRAKKE, MK
中科院分区：
文献类型：
--
作者：
HSU, YH;BRAKKE, MK
土传小麦花叶病毒(SBWMV)基因组由2个RNA种组成。3个菌株具有相同的大RNA，命名为1.0L RNA，但较小的RNA的大小不同，野生型(WT)为0.5L RNA，突变体Lab 2为0.4L RNA，突变Lab 1为0.35L RNA，其中L对应于.apprx。6700个核苷酸残基。兔网织红细胞裂解物中1.0L RNA的主要翻译产物的表观分子量分别为180000(180K)、152K、135K、80K和45K。这些都不是用抗血清病毒粒子沉淀的。0.5L RNA刺激90K、28K和19.7K产物的合成，0.4L RNA刺激66K、28K和19.7K产物的合成，0.35L RNA刺激55K、28K和19.7K产物的合成。19.7K蛋白与病毒外壳蛋白共迁移，是所有病例的主要产物。免疫沉淀、肽图和产物出现的时间进程表明，RNA II(0.5L、0.4L和0.35L RNA)的较大产物来自于通读。产物的模式与0.5L RNA 3‘’区的内部缺失形成0.4L和0.35L相一致。侵染SBWMV-WT小麦的提取物含有与0.5L RNA翻译产物在凝胶迁移率和免疫反应性上相对应的多肽。
The genome of soil-borne wheat mosaic (SBWMV) virus appears to be composed to 2 RNA species. Three strains have the same large RNA, designated 1.0L RNA, but differ in the size of the smaller RNA, designated 0.5L RNA for wild type (WT), 0.4L RNA for mutant Lab 2, and 0.35L RNA for mutant Lab 1, where L corresponds to .apprx. 6700 nucleotide residues. The major translation products of 1.0L RNA in rabbit reticulocyte lysates had apparent MW of 180,000 (180K), 152K, 135K, 80K and 45K. None of these were precipitated with antiserum virions. The 0.5L RNA stimulated the synthesis of products of 90K, 28K, and 19.7K, the 0.4L RNA of 66K, 28K, and 19.7K, the 0.35L RNA of 55K, 28K and 19.7K. Protein of 19.7K comigrated with viral coat protein and was the predominant product in all cases. Immunoprecipitation, peptide mapping, and the time course of appearance of products suggest that the larger products of RNA II (0.5L, 0.4L and 0.35L RNA) arise from readthrough. The pattern of products is consistent with formation of 0.4L and 0.35L by internal deletions in the 3'' region of 0.5L RNA. Extracts of SBWMV-WT-infected wheat contained polypeptides that corresponded to the translation products of 0.5L RNA in electrophoretic mobility and immunological reactivity.