Goose RIG-I functions in innate immunity against Newcastle disease virus infections
Goose RIG-I functions in innate immunity against Newcastle disease virus infections
复制标题
鹅 RIG-I 在针对新城疫病毒感染的先天免疫中发挥作用
DOI：
10.1016/j.molimm.2012.08.022
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发表时间：
2013-04-01
影响因子：
3.6
通讯作者：
Ding, Chan
中科院分区：
文献类型：
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作者：
Sun, Yingjie;Ding, Na;Ding, Chan
哺乳动物维甲酸诱导基因I(RIG-I)是包括新城疫病毒(NDV)在内的病毒RNA分子的主要抗病毒基因。本研究对鹅Rig-I基因(Grig-I)进行了鉴定。该基因全长2805bp，编码一个Grig-I蛋白，与鸭Rig-I的氨基酸同源性为93.8%。转染全长Grig-I或Grig-I的CARD结构域的DF-1鸡成纤维细胞对21-mer 5‘PPP RNA激动剂有显著的反应，表现为干扰素-β启动子活性的增强。然后检测转染鹅RIG-I基因的293T/17细胞对新城疫病毒感染的反应，结果表明，新城疫病毒感染后，干扰素-β启动子活性上调，IRF-3和IFIT1的mRNA水平升高，但病毒滴度下降。在新城疫病毒感染后，转基因的DF-1鸡成纤维细胞和鹅胚胎成纤维细胞也得到了类似的结果。动物实验进一步支持了Grig-I在鹅天然免疫中的作用，感染后鹅肺和气囊中的Grig-I mRNA水平升高，病毒滴度降低。(C)2012爱思唯尔有限公司。保留所有权利。
Mammalian retinoic acid-inducible gene I (RIG-I) is a chief antiviral gene sensing viral RNA molecules including Newcastle disease virus (NDV). In this study, goose RIG-I gene (gRIG-I) was identified. The 2805 bp-long gene encodes a gRIG-I protein that exhibits 93.8% amino acid identity to duck RIG-I. DF-1 chicken fibroblast cells transfected with full-length of gRIG-I or CARD domain of gRIG-I plasmids respond significantly to the agonist of 21-mer 5'ppp RNA, evident through enhancement of IFN-beta promoter activity. Goose RIG-I transfected 293T/17 cells were then tested for the response to NDV infection, resulting in up-regulated activity of IFN-beta promoter, and mRNA levels of IRF-3 and IFIT1, but decreased virus titer. Similar results were obtained in transfected DF-1 chicken fibroblast cells and goose embryo fibroblast cells in response to NDV infections Animal experiments further support a role of gRIG-I in goose innate immunity against NDV infections by showing increased gRIG-I mRNA levels and decreased virus titer in geese lung and air sac post-infection. (C) 2012 Elsevier Ltd. All rights reserved.