Polarization imaging and classification of Jurkat T and Ramos B cells using a flow cytometer
Polarization imaging and classification of Jurkat T and Ramos B cells using a flow cytometer
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使用流式细胞仪对 Jurkat T 和 Ramos B 细胞进行偏振成像和分类
DOI：
10.1002/cyto.a.22524
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Xin
中科院分区：
文献类型：
--
作者：
Yuanming Feng;Ning Zhang;K. Jacobs;Wenhuan Jiang;Li V. Yang;Zhigang Li;Jun Zhang;Jun Q. Lu;Xin
泡孔体积Vc B mm 663.7 6 308 666.5 6 296 0.962泡孔表面积Sc c mm 526.4 6 162 521.3 6 153 0.867泡孔表面积与体积比SVrc mm 21 0.8495 6 0.168 0.8493 6 0.207 0.996泡孔表面不规则指数ISIc d mm 201.7 6 28.5 199.7 6 20.6 0.678细胞膜体素到质心的平均距离mm 6.797 6 2.14 7.110 6 2.45 0.495 Rc的标准差DRc mm 1.841 6 0.721 2.051 6 0.854 0.186核体积Vn mm 3 407.8 6 <Rc>199 367.4 6 185 0.285细胞核表面积Sn mm 2 425.5 6 158 388.2 6 130 0.187细胞核表面不规则指数IS单位mm 21/2 206.8 6 42.1 204.3 6 28.9 0.716线粒体体积Vm mm 3 33.40 6 38.6 32.1 6 34.8 0.853线粒体表面积Sm mm 2 578.3 6 803 485.5 6 728 0.538线粒体表面积与体积比SVrm mm 21 13.56 6 3.80 12.48 6 2.84 0.100线粒体表面不规则指数ISIm mm 21/2 731.3 6 695 677.0 6 550 0.656细胞核与细胞重心之间的距离Dnc mm 0.1760 6 0.053 0.1597 6 0.048 0.096细胞核与细胞的体积比Vrnc 0.6280 6 0.126 0.5479 6 0.143 0.004电池体积比Vrmc 0.0526 6 0.066 0.0484 6 0.055 0.662
Cell volume Vc b mm 663.7 6 308 666.5 6 296 0.962 Cell surface area Sc c mm 526.4 6 162 521.3 6 153 0.867 Surface to volume ratio of cell SVrc mm 21 0.8495 6 0.168 0.8493 6 0.207 0.996 Index of surface irregularity of cell ISIc d mm 201.7 6 28.5 199.7 6 20.6 0.678 Average distance of cell membrane voxels to centroid <Rc> mm 6.797 6 2.14 7.110 6 2.45 0.495 Standard deviation of Rc DRc mm 1.841 6 0.721 2.051 6 0.854 0.186 Nuclear volume Vn mm 3 407.8 6 199 367.4 6 185 0.285 Nuclear surface area Sn mm 2 425.5 6 158 388.2 6 130 0.187 Index of surface irregularity of nucleus ISIn mm 21/2 206.8 6 42.1 204.3 6 28.9 0.716 Mitochondrial volume Vm mm 3 33.40 6 38.6 32.1 6 34.8 0.853 Mitochondrial surface area Sm mm 2 578.3 6 803 485.5 6 728 0.538 Surface to volume ratio of mitochondria SVrm mm 21 13.56 6 3.80 12.48 6 2.84 0.100 Index of surface irregularity of mitochondria ISIm mm 21/2 731.3 6 695 677.0 6 550 0.656 Distance between the centroids of nucleus and cell Dnc mm 0.1760 6 0.053 0.1597 6 0.048 0.096 Volume ratio of nucleus to cell Vrnc 0.6280 6 0.126 0.5479 6 0.143 0.004 Volume ratio of mitochondrion to cell Vrmc 0.0526 6 0.066 0.0484 6 0.055 0.662