MAGNETIC-RESONANCE MICROSCOPY OF MOUSE EMBRYOS
MAGNETIC-RESONANCE MICROSCOPY OF MOUSE EMBRYOS
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DOI：
10.1073/pnas.91.9.3530
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发表时间：
1994-04-26
影响因子：
11.1
通讯作者：
LINNEY, E
中科院分区：
文献类型：
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作者：
SMITH, BR;JOHNSON, GA;LINNEY, E
人们越来越多地使用小鼠作为哺乳动物生物学各个方面的模型，这引起了人们对制定检查和比较正常和异常小鼠胚胎发育和解剖学策略的新兴趣。在这项研究中，我们探索了使用磁共振显微镜作为实现这些目的的工具。描述了小鼠胚胎的固定、包埋、灌注和图像采集技术。牛血清白蛋白-二亚乙基三胺五乙酸酐钆作为造影剂的灌注增强了器官发生关键阶段发育中胚胎脉管系统的图像，并且允许在用视黄酸等致畸剂处理胚胎时进行比较。获取的三维数据集可用于存档、分发和采集后操作，例如解剖结构的计算机分割。
The increased use of the mouse as a model for various aspects of mammalian biology has caused a renewed interest in developing strategies for examining and comparing normal and abnormal mouse embryonic development and anatomy. In this study, we have explored the use of magnetic resonance microscopy as a tool for these purposes. Techniques for the fixation, embedding, perfusion, and image acquisition of mouse embryos are described. The perfusion of bovine serum albumin-diethylenetriaminepentaacetic anhydridegadolinium as a contrast agent enhances images of the developing embryonic vasculature during critical stages of organogenesis and allows for comparisons when embryos have been treated with teratogens such as retinoic acid. The acquired three-dimensional data sets are available for archiving, distributing, and postacquisition manipulations such as computer segmentation of anatomical structures.