Quantitative in vivo islet potency assay in normoglycemic nude mice correlates with primary graft function after clinical transplantation
Quantitative in vivo islet potency assay in normoglycemic nude mice correlates with primary graft function after clinical transplantation
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DOI：
10.1097/tp.0b013e31817ef846
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发表时间：
2008-07-27
期刊：
影响因子：
6.2
通讯作者：
Kerr-Conte, Julie
中科院分区：
文献类型：
--
作者：
Caiazzo, Robert;Gmyr, Valery;Kerr-Conte, Julie
在胰岛移植(IT)中，迫切需要可靠的检测方法来监测移植物的效力。我们测试了一种基于人C肽(HCP)测量的体内胰岛活性定量分析方法，该方法基于肾胶囊下给药后血糖正常的裸鼠。QIVIPA最初是通过移植增量剂量的人胰岛进行测试的。小鼠体内HCP水平与移植胰岛数量呈正相关(r(2)=0.6，P
Reliable assays are critically needed to monitor graft potency in islet transplantation (IT). We tested a quantitative in vivo islet potency assay QIVIPA) based on human C-peptide (hCP) measurements in normoglycemic nude mice after IT under the kidney capsule. QIVIPA was initially tested by transplanting incremental doses of human islets. hCP levels in mice were correlated with the number of transplanted islet equivalents (r(2) = 0.6, P