ONSET OF FIBER DIFFERENTIATION IN CULTURED RAT LENS EPITHELIUM UNDER THE INFLUENCE OF NEURAL RETINA-CONDITIONED MEDIUM
ONSET OF FIBER DIFFERENTIATION IN CULTURED RAT LENS EPITHELIUM UNDER THE INFLUENCE OF NEURAL RETINA-CONDITIONED MEDIUM
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DOI：
10.1016/0014-4835(84)90117-9
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发表时间：
1984-01-01
影响因子：
3.4
通讯作者：
MCAVOY, JW
中科院分区：
文献类型：
--
作者：
CAMPBELL, MT;MCAVOY, JW
在完整的大鼠晶状体中，长方体的含有α-晶体蛋白的上皮细胞产生纤维细胞，纤维细胞被拉长，含有α-晶体蛋白、β-晶体蛋白和γ-晶体蛋白。在牛神经视网膜(BRCM)条件培养液中培养的上皮外植体在体内表现出类似于纤维细胞分化的变化。细胞变大、拉长，并积聚β-和γ-晶体蛋白以及α-晶体蛋白。用[35S]-蛋氨酸标记的研究表明，在BRCM培养过程中，所有3类晶体蛋白的合成都发生了顺序的变化。2天后，α-晶体蛋白亚基的合成，特别是αA和αAINS的合成，相对于总的蛋白质合成增加。在培养4天后，首次检测到被鉴定为βB1a、βB4、βB5和可能的βA2的β-晶体蛋白亚基的合成，并且在4-8天之间可以检测到β-晶体蛋白的合成。γ-晶状体蛋白合成的开始时间似乎比β-晶状体蛋白合成的开始时间显示出更大的实验变异性。在BRCM.apprx中培养10天的外植体。25%的新蛋白质合成可以归因于α-、β-和伽马-晶体蛋白。这些事件完全依赖于BRCM，这表明神经视网膜分泌一种启动纤维细胞分化的因子(S)。该培养体系适合于研究纤维分化的体外调控。
In the intact rat lens, epithelial cells, which are cuboidal and contain .alpha.-crystallin, give rise to fiber cells, which are elongated and contain .alpha.-, .beta.- and .gamma.-crystallins. Epithelial explants cultured in medium conditioned by bovine neural retinas (BRCM) showed changes analogous to fiber cell differentiation in vivo. The cells became enlarged and elongated and accumulated .beta.- and .gamma.-crystallin as well as .alpha.-crystallin. Labeling studies with [35S]-methionine showed that sequential changes in the synthesis of all 3 classes of crystallin occurred during culture in BRCM. After 2 days, synthesis of .alpha.-crystallin subunits, particularly .alpha.A and .alpha.AINS, increased relative to overall protein synthesis. After 4 days in culture, synthesis of .beta.-crystallin subunits identified as .beta.B1a, .beta.B4, .beta.B5 and possibly .beta.A2, was detected for the first time and between 4 and 8 days .gamma.-crystallin synthesis became detectable. The time of onset of .gamma.-crystallin synthesis seemed to show greater experimental variability than did onset of .beta.-crystallin synthesis. In explants cultured for 10 days in BRCM .apprx. 25% of new protein synthesis could be attributed to .alpha.-, .beta.- and .gamma.-crystallins. These events were completely dependent on BRCM, suggesting that neural retina secretes a factor(s) which initiates fiber cell differentiation. This culture system appears to be a suitable one for investigating the control of fiber differentiation in vitro.