Reactive oxygen species regulate protrusion efficiency by controlling actin dynamics.
Reactive oxygen species regulate protrusion efficiency by controlling actin dynamics.
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DOI：
10.1371/journal.pone.0041342
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
DerMardirossian C
中科院分区：
文献类型：
--
作者：
Taulet N;Delorme-Walker VD;DerMardirossian C
Productive protrusions allowing motile cells to sense and migrate toward a chemotactic gradient of reactive oxygen species (ROS) require a tight control of the actin cytoskeleton. However, the mechanisms of how ROS affect cell protrusion and actin dynamics are not well elucidated yet. We show here that ROS induce the formation of a persistent protrusion. In migrating epithelial cells, protrusion of the leading edge requires the precise regulation of the lamellipodium and lamella F-actin networks. Using fluorescent speckle microscopy, we showed that, upon ROS stimulation, the F-actin retrograde flow is enhanced in the lamellipodium. This event coincides with an increase of cofilin activity, free barbed ends formation, Arp2/3 recruitment, and ERK activity at the cell edge. In addition, we observed an acceleration of the F-actin flow in the lamella of ROS-stimulated cells, which correlates with an enhancement of the cell contractility. Thus, this study demonstrates that ROS modulate both the lamellipodium and the lamella networks to control protrusion efficiency.
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影响因子：
16
作者：
Andrianantoandro, Ernesto;Pollard, Thomas D.
通讯作者：
Pollard, Thomas D.
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DOI：
10.1083/jcb.136.6.1307
发表时间：
1997-03-24
期刊：
The Journal of cell biology
影响因子：
--
作者：
Carlier MF;Laurent V;Santolini J;Melki R;Didry D;Xia GX;Hong Y;Chua NH;Pantaloni D
通讯作者：
Pantaloni D
影响因子：
15.9
作者：
Aikawa, R;Komuro, I;Yazaki, Y
通讯作者：
Yazaki, Y
影响因子：
3.4
作者：
DalleDonne, I;Milzani, A;Colombo, R
通讯作者：
Colombo, R
影响因子：
9.2
作者：
Dawe, HR;Minamide, LS;Cramer, LP
通讯作者：
Cramer, LP