Modulation of microtubule dynamic instability in vivo by brain microtubule associated proteins.
Modulation of microtubule dynamic instability in vivo by brain microtubule associated proteins.
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脑微管相关蛋白对体内微管动态不稳定性的调节。
DOI：
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发表时间：
1995
影响因子：
4
通讯作者：
Patricia Wadsworth
中科院分区：
文献类型：
--
作者：
Rama Dhamodharan;Patricia Wadsworth
将热稳定的脑微管相关蛋白（MAP）和纯化的微管相关蛋白2（MAP-2）显微注射到培养的BSC-1细胞中，所述BSC-1细胞预先注射了罗丹明标记的微管蛋白。然后使用微光荧光显微镜和定量微管跟踪方法检查单个微管的动态不稳定行为。这两种MAP制剂通过降低生长和缩短事件的平均速率和程度来抑制体内微管动力学。生长事件的平均持续时间没有受到影响。当测量为事件/单位时间时，热稳定的MAP和MAP-2并没有显著改变救援的频率;灾难的频率降低约2倍，热稳定的MAP和MAP-2。当转换频率计算为事件/单位距离时，两种MAP准备都增加了救援频率，而没有改变灾难的频率。测定了生长期、收缩期和停顿期所占总时间的百分比。MAP-2和热稳定MAP均降低缩短时间百分比，增加停顿时间百分比，对生长时间百分比无影响。热稳定的MAP增加了平均停顿时间，减少了每个微管每分钟的平均事件数，并增加了停顿微管转向生长而不是缩短的可能性。结果表明，添加MAPs活细胞减少了个别微管的动态行为，主要是通过抑制动态事件的幅度和增加暂停所花费的时间，其中没有变化的微管长度可以检测到。结果进一步表明，MAPs的表达直接有助于细胞类型特异性微管动力学行为。
Heat-stable brain microtubule associated proteins (MAPs) and purified microtubule associated protein 2 (MAP-2) were microinjected into cultured BSC-1 cells which had been previously injected with rhodamine-labeled tubulin. The dynamic instability behavior of individual microtubules was then examined using low-light-level fluorescence microscopy and quantitative microtubule tracking methods. Both MAP preparations suppressed microtubule dynamics in vivo, by reducing the average rate and extent of both growing and shortening events. The average duration of growing events was not affected. When measured as events/unit time, heat-stable MAPs and MAP-2 did not significantly alter the frequency of rescue; the frequency of catastrophe was decreased approximately two-fold by heat-stable MAPs and MAP-2. When transition frequencies were calculated as events/unit distance, both MAP preparations increased the frequency of rescue, without altering the frequency of catastrophe. The percentage of total time spent in the phases of growth, shrink and pause was determined. Both MAP-2 and heat-stable MAPs decreased the percentage of time spent shortening, increased the percentage of time spent paused, and had no effect on percentage of time spent growing. Heat-stable MAPs increased the average pause duration, decreased the average number of events per minute per microtubule and increased the probability that a paused microtubule would switch to growing rather than shortening. The results demonstrate that addition of MAPs to living cells reduces the dynamic behavior of individual microtubules primarily by suppressing the magnitude of dynamic events and increasing the time spent in pause, where no change in the microtubule length can be detected. The results further suggest that the expression of MAPs directly contributes to cell type-specific microtubule dynamic behavior.
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微管相关蛋白2与微管的平衡结合机制。
DOI：
--
发表时间：
1993
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Wallis,KT;Azhar,S;Rho,MB;Lewis,SA;Cowan,NJ;Murphy,DB
通讯作者：
Murphy,DB
影响因子：
4
作者：
Gloria Lee;Susan L. Rook
通讯作者：
Gloria Lee;Susan L. Rook
微管相关蛋白 2 改变微管组装和拆卸的动态特性。
DOI：
--
发表时间：
1993
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Kowalski,RJ;WilliamsJr,RC
通讯作者：
WilliamsJr,RC
来自培养的 HeLa 细胞的微管相关蛋白。
DOI：
--
发表时间：
1980
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Bulinski,JC;Borisy,GG
通讯作者：
Borisy,GG
影响因子：
2.9
作者：
R. Toso;M. Jordan;K. Farrell;B. Matsumoto;L. Wilson
通讯作者：
R. Toso;M. Jordan;K. Farrell;B. Matsumoto;L. Wilson