PHB3 interacts with BRI1 and BAK1 to mediate brassinosteroid signal transduction in Arabidopsis and tomato
PHB3 interacts with BRI1 and BAK1 to mediate brassinosteroid signal transduction in Arabidopsis and tomato
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DOI：
10.1111/nph.19469
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发表时间：
2023-12-21
期刊：
影响因子：
9.4
通讯作者：
Wang，Xiaofeng
中科院分区：
文献类型：
--
作者：
Li，Cheng;Zhang，Shan;Wang，Xiaofeng
类维生素B1（BRs）是植物生长发育所必需的激素。BRI 1和BAK 1位于细胞膜上，作为受体接受和传递BR信号。通过亲和纯化和LC-MS/MS分析，在BRI 1和BAK 1复合物中鉴定出抑制蛋白3（PHB 3）。生化数据表明，BRI 1/BAK 1在体外和体内均能与PHB 3相互作用，在体外BRI 1/BAK 1可使PHB 3磷酸化。当PHB 3中的Thr-80氨基酸突变为Ala时，突变蛋白不被BRI 1磷酸化，并且在酵母双杂交测定中消除了突变蛋白与BRI 1的相互作用。BAK 1不磷酸化突变蛋白PHB 3 T54 A。功能丧失的phb 3突变体显示出比野生型更弱的BR信号。遗传分析表明，PHB 3是BRI 1/BAK 1下游底物，参与BR信号传导。PHB 3在番茄中有5个纯合突变体，其中与AtPHB 3最接近的一个被命名为SlPHB3.1。生物化学数据显示SlBRI 1/SlSERK 3A/SlSERK 3B与SlPHB3.1和SlPHB3.3相互作用。通过CRISPR-Cas9方法获得的slphb 3. 1突变体导致番茄BR信号相对受阻，PHB 3是番茄和番茄BR信号途径的新组分。
Brassinosteroids (BRs) are plant hormones that are essential in plant growth and development. BRASSINOSTEROID‐INSENSITIVE 1 (BRI1) and BRI1 ASSOCIATED RECEPTOR KINASE 1 (BAK1), which are located on the plasma membrane, function as co‐receptors that accept and transmit BR signals.PROHIBITIN 3 (PHB3) was identified in both BRI1 and BAK1 complexes by affinity purification and LC‐MS/MS analysis. Biochemical data showed that BRI1/BAK1 interacted with PHB3in vitroandin vivo.BRI1/BAK1 phosphorylated PHB3in vitro. When the Thr‐80 amino acid in PHB3 was mutated to Ala, the mutant protein was not phosphorylated by BRI1 and the mutant protein interaction with BRI1 was abolished in the yeast two‐hybrid assay. BAK1 did not phosphorylate the mutant protein PHB3T54A. The loss‐of‐functionphb3mutant showed a weaker BR signal than the wild‐type. Genetic analyses revealed that PHB3 is a BRI1/BAK1 downstream substrate that participates in BR signalling. PHB3 has five homozygous in tomato, and we named the closest to AtPHB3 as SlPHB3.1. Biochemical data showed that SlBRI1/SlSERK3A/SlSERK3B interacted with SlPHB3.1 and SlPHB3.3. The CRISPR‐Cas9 method generatedslphb3.1mutant led to a BR signal stunted relatively in tomatoes.PHB3 is a new component of the BR signal pathway in bothArabidopsisand tomato.