SCRAPIE-ASSOCIATED PRION PROTEIN ACCUMULATES IN ASTROCYTES DURING SCRAPIE INFECTION
SCRAPIE-ASSOCIATED PRION PROTEIN ACCUMULATES IN ASTROCYTES DURING SCRAPIE INFECTION
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DOI：
10.1073/pnas.88.2.375
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发表时间：
1991-01-01
影响因子：
11.1
通讯作者：
HAASE, AT
中科院分区：
文献类型：
--
作者：
DIEDRICH, JF;BENDHEIM, PE;HAASE, AT
瘙痒病是一种由非常规因素引起的传染性海绵状脑病，在此过程中，正常的细胞蛋白会转化为异常形式，与感染性结合并聚集形成淀粉样蛋白沉积。我们使用免疫细胞化学和增强淀粉样蛋白检测的方法来研究中枢神经系统中参与异常瘙痒相关蛋白形成的细胞类型。我们发现，这种蛋白在星形胶质细胞蓄积之前，主要的神经病理改变为瘙痒-星形胶质细胞增生症、空泡化、神经元丢失和淀粉样蛋白沉积。这些发现表明星形胶质细胞参与了瘙痒病中PrP蛋白和淀粉样蛋白的瘙痒异构体的形成，并提示这种细胞类型可能也参与了瘙痒病病原体的复制。
In the course of scrapie, a transmissible spongiform encephalopathy caused by an unconventional agent, a normal cellular protein is converted to an abnormal form that copurifies with infectivity and aggregates to form deposits of amyloid. We have used immunocytochemistry and methods that enhance detection of amyloidogenic proteins to investigate the types of cells in the central nervous system which are involved in the formation of the abnormal scrapie-associated protein. We show that this protein accumulates in astrocytes prior to the cardinal neuropathological changes in scrapie-astrogliosis, vacuolation, neuron loss, and amyloid deposition. These findings implicate the astrocyte in the formation of the scrapie isoform of the prion protein and amyloid in scrapie and suggest that this cell type might also be involved in the replication of the scrapie agent.