Effects of retinoic acid and juvenile hormone on the induction of ornithine decarboxylase activity by 12-O-tetradecanoylphorbol-13-acetate.
Effects of retinoic acid and juvenile hormone on the induction of ornithine decarboxylase activity by 12-O-tetradecanoylphorbol-13-acetate.
复制标题
视黄酸和保幼激素对 12-O-十四烷酰佛波醇-13-乙酸酯诱导鸟氨酸脱羧酶活性的影响。
作者：
T. Kensler;A. Verma;R. Boutwell;G. Mueller
肿瘤促进剂12-O-十四烷酰佛波醇-13-乙酸酯（TPA），一种在植物血凝素处理的牛淋巴细胞中的高活性共分裂原，诱导鸟氨酸脱羧酶活性比单独用凝集素处理的培养物增加11倍。TPA的这种协同作用可以通过同时加入无环倍半萜、昆虫保幼激素III来拮抗。维甲酸（维生素A酸），TPA在小鼠中的肿瘤促进作用的抑制剂，也是一种有效的拮抗剂，但需要管理凝集素激活的淋巴细胞1小时前TPA。这些数据表明，代谢活化的视黄酸是必要的，以发挥其拮抗作用。淋巴细胞和小鼠皮肤中的反应的比较表明，淋巴细胞提供了一个很好的系统，通过它的分子过程，佛波酯和维甲酸的影响正常和癌前细胞的生长和分化的研究。
The tumor-promoting agent 12-O-tetradecanoylphorbol-13-acetate (TPA), a highly active comitogen in phytohemagglutinin-treated bovine lymphocytes, induces an 11-fold increase in ornithine decarboxylase activity over cultures treated with the lectin alone. This synergistic action of TPA could be antagonized by the simultaneous addition of the acyclic sesquiterpene, insect juvenile hormone III. Retinoic acid (vitamin A acid), an inhibitor of the tumor-promoting action of TPA in mice, was also an effective antagonist but required administration to lectin-activated lymphocytes 1 hr prior to TPA. These data suggest that metabolic activation of retinoic acid is required in order to exert its antagonistic action. Comparison of the responses in the lymphocytes and mouse skin suggests that the lymphocytes provide an excellent system for studying the molecular processes through which phorbol esters and retinoids influence the growth and differentiation of both normal and premalignant cells.