Adenine nucleotide content of liver mitochondria increases after glucagon treatment of rats or isolated hepatocytes.
Adenine nucleotide content of liver mitochondria increases after glucagon treatment of rats or isolated hepatocytes.
复制标题
胰高血糖素处理大鼠或分离的肝细胞后，肝线粒体的腺嘌呤核苷酸含量增加。
DOI：
10.1016/0006-291x(82)90905-6
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发表时间：
1982
影响因子：
3.1
通讯作者：
BrennanJr,WA
中科院分区：
文献类型：
--
作者：
Aprille,JR;Nosek,MT;BrennanJr,WA
通过尾静脉注射胰高血糖素或与体外分离的肝细胞一起孵育，可使线粒体内 ATP+ADP+AMP 含量增加 22-35%。这些线粒体的状态 3 或非耦合呼吸频率没有统计学上的显着变化。无论使用何种介质或分离线粒体所需的时间如何，都注意到类似的胰高血糖素效应。最近，关于评估胰高血糖素对线粒体功能的影响时可能出现的伪影存在争议。目前的结果表明，胰高血糖素诱导的基质腺嘌呤核苷酸含量增加是一种可重复的现象，具有可能的生理意义。
Glucagon injected into rats via tail vein or incubated with isolated hepatocytesin vitrocaused a 22–35% increase in the intramitochondrial ATP+ADP+AMP content. There was no statistically significant change in state 3 or uncoupled respiratory rates in these mitochondria. Similar glucagon effects were noted irrespective of the medium used or time required for isolation of mitochondria. There has been recent controversy over possible artifacts in assessing the effects of glucagon on mitochondrial function. The present results suggest that the glucagon-induced increase in the matrix adenine nucleotide content is a reproducible phenomenon with probable physiological significance.